Oishi Peter E, Cholsiripunlert Sompob, Gong Wenhui, Baker Anthony J, Bernstein Harold S
Cardiovascular Research Institute, University of California San Francisco, USA.
J Vis Exp. 2011 Feb 22(48):2582. doi: 10.3791/2582.
To assess the in vivo effects of therapeutic interventions for the treatment of muscle disease, quantitative methods are needed that measure force generation and fatigability in treated muscle. We describe a detailed approach to evaluating myo-mechanical properties in freshly explanted hindlimb muscle from the mouse. We describe the atraumatic harvest of mouse extensor digitorum longus muscle, mounting the muscle in a muscle strip myograph (Model 820MS; Danish Myo Technology), and the measurement of maximal twitch and tetanic tension, contraction time, and half-relaxation time, using a square pulse stimulator (Model S48; Grass Technologies). Using these measurements, we demonstrate the calculation of specific twitch and tetanic tension normalized to muscle cross-sectional area, the twitch-to-tetanic tension ratio, the force-frequency relationship curve and the low frequency fatigue curve. This analysis provides a method for quantitative comparison between therapeutic interventions in mouse models of muscle disease, as well as comparison of the effects of genetic modification on muscle function.
为了评估治疗性干预措施对肌肉疾病治疗的体内效果，需要定量方法来测量治疗后肌肉的力量产生和疲劳性。我们描述了一种详细的方法，用于评估从小鼠新鲜取出的后肢肌肉的肌-力学特性。我们描述了无创伤获取小鼠趾长伸肌的方法，将肌肉安装在肌肉条肌动描记器（820MS型；丹麦肌技术公司）中，并使用方波刺激器（S48型；格拉斯技术公司）测量最大单收缩和强直张力、收缩时间和半松弛时间。通过这些测量，我们展示了特定单收缩和强直张力相对于肌肉横截面积的计算、单收缩与强直张力之比、力-频率关系曲线和低频疲劳曲线。该分析提供了一种方法，可用于在肌肉疾病小鼠模型中对治疗性干预措施进行定量比较，以及比较基因修饰对肌肉功能的影响。
