Najid A, Habrioux G
Laboratoire de Biochimie, Faculté de Pharmacie, Université de Limoges, France.
Oncology. 1990;47(3):269-74. doi: 10.1159/000226830.
The MCF-7 human breast cancer cell line responds to 5-androstene-3 beta,17 beta-diol, to dehydroepiandrosterone and to its 3 beta-sulfate stimulation in vitro by increased proliferation. 5-Androstene-3 beta,17 beta-diol has the strongest mitogenic effect. Dehydroepiandrosterone has a more potent action on cell proliferation than its 3 beta-sulfate. These adrenal androgens do not stimulate proliferation of the estrogen receptors negative for the BT-20 cell line. In our conditions, we have failed to demonstrate MCF-7 estradiol formation when incubations were carried out in the presence of labeled adrenal androgens. These results suggest that adrenal androgens stimulate proliferation of MCF-7 cells directly by acting on estrogen receptors.
MCF - 7人乳腺癌细胞系在体外对5 - 雄烯 - 3β,17β - 二醇、脱氢表雄酮及其3β - 硫酸盐刺激有反应，表现为增殖增加。5 - 雄烯 - 3β,17β - 二醇具有最强的促有丝分裂作用。脱氢表雄酮对细胞增殖的作用比其3β - 硫酸盐更强。这些肾上腺雄激素不会刺激雌激素受体阴性的BT - 20细胞系的增殖。在我们的实验条件下，当在标记的肾上腺雄激素存在下进行孵育时，我们未能证明MCF - 7形成雌二醇。这些结果表明，肾上腺雄激素通过作用于雌激素受体直接刺激MCF - 7细胞的增殖。
