Hamasuna Ryoichi, Kawai Shuichi, Ando Yukiko, Ito Kenji, Kurashima Motoko, Nishimura Hirohumi, Yamaguchi Takamasa, Yoshimura Makoto, Kobayashi Tomoko, Muratani Tetsuro, Matsumoto Tetsuro
Department of Urology, University of Occupational and Environmental Health.
Kansenshogaku Zasshi. 2011 Jan;85(1):1-7. doi: 10.11150/kansenshogakuzasshi.85.1.
We evaluated performance of Abbott RealTime CT/NG assay (real-time PCR, Abbott Japan) for detect Chlamydia trachomatis and Neisseria gonorrhoeae by real-time PCR in 88 female patients with cervicitis symptoms seen at gynecological clinics and 100 male patients with urethritis symptoms seen at urological or dermatology clinics in Kitakyushu, Japan. Endocervical swab and first-voided urine (FVU) specimens were then collected from women and FVU specimens from men. Detection rates of C. trachomatis and N. gonorrhoeae by real-time PCR in the 3 types of specimens were compared to those by ProbeTec ET assay (ProbeTec, BD Diagnostic System). The overall positive concordance between real-time PCR and ProbTec were 97.1% (66/68) for C. trachomatis and 100% (33/33) for N. gonorrhoeae, C. trachomatis detection yielded 3 discordant results in endocervical specimens and 1 discordant result in male FVU by real-time PCR and ProbTec. Three of 4 reexamined using Aptime Combo 2 Assay (Fuji Rebio Inc.) were positive for C. trachomatis. Endocervical swab and FVU specimen results for C. trachomatis were discordant in 3 cases in real-time PCR and 4 in ProbeTec. Subjects with 2 or more positive endocervical awab results in female or male FVU specimens were assumed to be "true positive" for C. trachomatis. The sensitivities of real-time PCR for detecting C. trachomatis was 94.4% in endocervical swabs, 77.8% in female FVU and 97.4% in the male FVU. The sensitivities for real-time PCR for detecting N. gonorrhoeae was 100% in all 3 specimentypes. Abbott RealTime CT/NG assay was useful for detecting C. trachomatis using endocervical swabs or male FVU specimens and for detecting N. gonorrhoeae using endocervical swabs and all FVU specimens.
我们评估了雅培实时CT/NG检测法（实时PCR，雅培日本公司）在日本北九州妇科诊所就诊的88例有宫颈炎症状的女性患者以及泌尿科或皮肤科诊所就诊的100例有尿道炎症状的男性患者中，通过实时PCR检测沙眼衣原体和淋病奈瑟菌的性能。然后从女性患者中采集宫颈拭子和首次晨尿（FVU）样本，从男性患者中采集FVU样本。将3种类型样本中实时PCR检测沙眼衣原体和淋病奈瑟菌的检出率与ProbeTec ET检测法（ProbeTec，BD诊断系统）的检出率进行比较。实时PCR与ProbTec之间，沙眼衣原体的总体阳性一致性为97.1%（66/68），淋病奈瑟菌为100%（33/33），实时PCR和ProbTec检测沙眼衣原体时，在宫颈标本中有3个不一致结果，在男性FVU中有1个不一致结果。使用Aptime Combo 2检测法（富士瑞必欧株式会社）重新检测的4例中有3例沙眼衣原体呈阳性。实时PCR检测沙眼衣原体时，宫颈拭子和FVU样本结果在其中3例中不一致，ProbeTec检测时在4例中不一致。女性宫颈拭子或男性FVU样本中有2个或更多阳性结果的受试者被假定为沙眼衣原体“真阳性”。实时PCR检测沙眼衣原体的灵敏度在宫颈拭子中为94.4%，在女性FVU中为77.8%，在男性FVU中为97.4%。实时PCR检测淋病奈瑟菌的灵敏度在所有3种样本类型中均为100%。雅培实时CT/NG检测法可用于使用宫颈拭子或男性FVU样本检测沙眼衣原体，以及使用宫颈拭子和所有FVU样本检测淋病奈瑟菌。
