Zhou Yu-Yang, Chen Dong-Zhi, Jin Xiao-Jun, Chen Jian-Meng, He Jie
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
Huan Jing Ke Xue. 2011 Jan;32(1):266-71.
A tetrahydrofuran (THF)-degrading strain Pseudomonas oleovorans DT4 was isolated from the activated sludge of a pharmaceutical plant. P. oleovorans DT4 was able to utilize THF as the sole carbon and energy source under aerobic condition. 5 mmol/L of THF could be completely degraded by 3.2 mg/L inoculums of P. oleovorans DT4 in 14 h at pH 7.2 and 30 degrees C, with the cells concentration increasing to 188.6 mg/L. After the complete consumption of THF, no TOC could be detected but IC reached the stable value of about 46 mg/L, with pH decreasing to 6.54, which indicated that the substance was totally mineralized by P. oleovorans DT4. The optimum conditions for THF biodegradation in shaking flasks were pH 7.5 and temperature 37 degrees C, respectively. Results from the oxygen control experiments revealed that the oxygen supply by shaking was the satisfactory growth condition. Additionally, as the important elements for DT4, Mg2+ and Ca2+ at concentrations of 0.80 mmol/L and 0.20 mmol/L, respectively, were suitable for THF degradation. All the results contribute to the efficient bioremediation for the THF contaminated.
从一家制药厂的活性污泥中分离出一株能够降解四氢呋喃（THF）的菌株——食油假单胞菌DT4。食油假单胞菌DT4在有氧条件下能够利用THF作为唯一的碳源和能源。在pH值为7.2、温度为30℃的条件下，3.2 mg/L的食油假单胞菌DT4接种物能够在14小时内将5 mmol/L的THF完全降解，细胞浓度增加到188.6 mg/L。THF完全消耗后，未检测到总有机碳（TOC），但无机碳（IC）达到约46 mg/L的稳定值，pH值降至6.54，这表明该物质已被食油假单胞菌DT4完全矿化。摇瓶中THF生物降解的最佳条件分别为pH值7.5和温度37℃。氧气控制实验结果表明，振荡供氧是理想的生长条件。此外，作为DT4的重要元素，浓度分别为0.80 mmol/L的Mg2+和0.20 mmol/L的Ca2+适合THF降解。所有这些结果都有助于对受THF污染的环境进行高效生物修复。
