Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM University, Kattankulathur-603 203, India.
Acta Pharm. 2011 Mar;61(1):37-50. doi: 10.2478/v10007-011-0007-5.
Simultaneous determination of valsartan and hydrochlorothiazide by the H-point standard additions method (HPSAM) and partial least squares (PLS) calibration is described. Absorbances at a pair of wavelengths, 216 and 228 nm, were monitored with the addition of standard solutions of valsartan. Results of applying HPSAM showed that valsartan and hydrochlorothiazide can be determined simultaneously at concentration ratios varying from 20:1 to 1:15 in a mixed sample. The proposed PLS method does not require chemical separation and spectral graphical procedures for quantitative resolution of mixtures containing the titled compounds. The calibration model was based on absorption spectra in the 200-350 nm range for 25 different mixtures of valsartan and hydrochlorothiazide. Calibration matrices contained 0.5-3 μg mL-1 of both valsartan and hydrochlorothiazide. The standard error of prediction (SEP) for valsartan and hydrochlorothiazide was 0.020 and 0.038 μg mL-1, respectively. Both proposed methods were successfully applied to the determination of valsartan and hydrochlorothiazide in several synthetic and real matrix samples.
本文描述了使用 H 点标准加入法（HPSAM）和偏最小二乘法（PLS）校准同时测定缬沙坦和氢氯噻嗪的方法。在加入缬沙坦标准溶液的情况下，监测 216nm 和 228nm 处的吸光度。应用 HPSAM 的结果表明，在混合样品中，缬沙坦和氢氯噻嗪的浓度比可以在 20:1 到 1:15 之间同时测定。所提出的 PLS 方法不需要化学分离和光谱图形程序即可定量解析含有标题化合物的混合物。校准模型基于 25 种不同的缬沙坦和氢氯噻嗪混合物在 200-350nm 范围内的吸收光谱。校准矩阵包含 0.5-3μg mL-1 的缬沙坦和氢氯噻嗪。缬沙坦和氢氯噻嗪的预测标准误差（SEP）分别为 0.020 和 0.038μg mL-1。所提出的两种方法均成功应用于几种合成和实际基质样品中缬沙坦和氢氯噻嗪的测定。
