Han D, Xue S
Institute of Basic Medical Sciences, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1990 Feb;12(1):74-8.
Plasmid PSV2-neo DNA containing neo gene (PSV2 neo) was successfully transferred into Wg3h cells, a HGPRT-Chinese hamster fibroblast cell line, by calcium phosphate DNA co-precipitating technique. Cells carrying two selectable markers (AGR and G418R) were thus obtained and passed for more than 40 generations, and designated as Wg3h-neo cell line. The existence of PSV2-neo sequences in the Wg3h-neo genome was confirmed by dot-blot hybridization with PSV2-neo DNA probe. Comparative studies showed that no significant difference in morphology, growth rate or genetic characteristics could be found between the Wg3h and Wg3h-neo cell lines.
含有新霉素基因（PSV2 neo）的质粒PSV2-neo DNA通过磷酸钙-DNA共沉淀技术成功导入Wg3h细胞，这是一种次黄嘌呤鸟嘌呤磷酸核糖转移酶缺陷型中国仓鼠成纤维细胞系。由此获得了携带两种选择标记（AGR和G418R）的细胞，并传代40多代，命名为Wg3h-neo细胞系。通过用PSV2-neo DNA探针进行斑点杂交，证实了Wg3h-neo基因组中PSV2-neo序列的存在。比较研究表明，Wg3h和Wg3h-neo细胞系在形态、生长速率或遗传特征方面未发现显著差异。
