Mourek J
Fyziologický ústav fakulty vseobecného lékarství Univerzity Karlovy, Praha.
Sb Lek. 1990 Mar;92(2-3):71-7.
On five-day-old and adult Wistar rats (of both sexes) experiments were made where the influence of the pH of the medium was assessed as well as that of a lower ATP supply on the activity of (Na(+)-K+) and Mg++ dependent ATPases (E.C.3.6.1.3.) isolated from tissue of the cerebral cortex and possibly the medulla oblongata. The enzyme activity is expressed in mumol.l-1 of released Pi (minute/mg protein). It was found that the drop of pH of the incubation medium (adjusted with HCl) to 7.0 and 6.4 (as compared with pH 7.4 in control measurements) did not cause in 5-day-old rats a significant change in the activity of ouabain sensitive ATPase of the cerebral cortex or medulla oblongata, while in adult rats a marked reduction was observed. At pH 6.4 in adult rats a greater drop of the activity in the cerebral cortex than in tissue of the medulla oblongata was demonstrated. Equally resistant was also ouabain sensitive ATPase in 5-day old rats against the ATP concentration of the medium; even a drop by 50% (as compared with control values) did not cause a significant reduction of activity, while in adult rats a significant drop was observed. In ouabain sensitive ATPase the position is different. In 5-day-old rats a lower pH reduces its activity to an equal extent for pH 7.0 and pH 6.4. In adult rats the activity is also reduced, the reduction being the more marked the more acid the pH of the medium (cerebral cortex).(ABSTRACT TRUNCATED AT 250 WORDS)
在5日龄和成年（雌雄皆有）的Wistar大鼠身上进行了实验，评估了培养基pH值以及较低的ATP供应对从大脑皮层组织以及可能从延髓分离出的（Na(+)-K+）和Mg++依赖性ATP酶（E.C.3.6.1.3.）活性的影响。酶活性以释放的Pi的微摩尔每升（分钟/毫克蛋白质）表示。结果发现，将孵育培养基的pH值（用HCl调节）降至7.0和6.4（与对照测量中的pH 7.4相比），在5日龄大鼠中，大脑皮层或延髓的哇巴因敏感ATP酶活性没有显著变化，而在成年大鼠中则观察到明显降低。在成年大鼠中，pH 6.4时大脑皮层的活性下降幅度大于延髓组织。5日龄大鼠的哇巴因敏感ATP酶对培养基的ATP浓度也同样具有抗性；即使下降50%（与对照值相比）也不会导致活性显著降低，而在成年大鼠中则观察到显著下降。在哇巴因敏感ATP酶方面情况不同。在5日龄大鼠中，较低的pH值在pH 7.0和pH 6.4时同等程度地降低其活性。在成年大鼠中活性也会降低，培养基的pH值越酸（大脑皮层），降低越明显。（摘要截短至250字）
