Whelan J, O'Mahony P, Harmey M A
Department of Botany, University College Dublin, Belfield, Ireland.
Arch Biochem Biophys. 1990 Jun;279(2):281-5. doi: 10.1016/0003-9861(90)90492-h.
Precursor proteins from Neurospora crassa were correctly processed by a matrix extract from Vicia faba and cauliflower mitochondria. Processing yielded mature protein of the same molecular mass as mature Neurospora protein. The processing activity has two components. One is antigenically related to and of the same molecular mass as the processing enhancing protein of Neurospora. The second component was not recognized by antibody to the matrix processing protease from Neurospora mitochondria. The second component also houses the protease activity. Similar results were obtained using precursors to both the F1 beta subunit of the mitochondrial F0F1 ATPase and subunit V of the Rieske FeS complex from Neurospora. The beta subunit of the F0F1 ATPASE was processed to the mature form. Subunit V of the Rieske FeS complex was processed to the intermediate form only. Additional processing seen during import into plant mitochondria is not catalyzed by these proteins.
粗糙脉孢菌的前体蛋白能够被蚕豆和花椰菜线粒体的基质提取物正确加工。加工后产生的成熟蛋白与成熟的粗糙脉孢菌蛋白具有相同的分子量。这种加工活性有两个组分。一个在抗原性上与粗糙脉孢菌的加工增强蛋白相关且分子量相同。第二个组分不能被针对粗糙脉孢菌线粒体基质加工蛋白酶的抗体识别。第二个组分也具有蛋白酶活性。使用粗糙脉孢菌线粒体F0F1 ATP酶的F1β亚基和 Rieske FeS复合物的V亚基的前体也获得了类似结果。F0F1 ATP酶的β亚基被加工成成熟形式。Rieske FeS复合物的V亚基仅被加工成中间形式。这些蛋白不会催化导入植物线粒体过程中出现的额外加工。
