C.N.R.S., Institut de Microbiologie de la Méditerranée, Aix-Marseille Université, Laboratoire de Chimie Bactérienne, UPR9043, 31 chemin Joseph Aiguier, 13402, Marseille, France.
Arch Microbiol. 2011 Jun;193(6):439-49. doi: 10.1007/s00203-011-0684-y. Epub 2011 Mar 16.
A moderately acidophilic, facultative chemoautotrophic, As(III)-oxidizing Thiomonas sp. (strain 3As(T)) was previously shown, on the basis of comparative 16S rRNA gene sequences, to be closely related to both Tm. perometabolis DSM 18570(T) and Tm. intermedia DSM 18155(T). While it had shared many physiological traits with Tm. intermedia (T), a mean DNA-DNA hybridization value (DDHV) of 47.2% confirmed that strain 3As(T) was not a strain of Tm. intermedia, though the situation with regard to Tm. perometabolis (DDHV previously determined as 72%) was more ambiguous. A comparative physiological and chemotaxonomic study of strain 3As(T) and Tm. perometabolis (T) was therefore carried out, together with multilocus sequence analysis (MLSA) of all three bacteria. Differences in fatty acid profiles and utilization of organic substrates supported the view that strain 3As(T) and Tm. perometabolis are distinct species, while MLSA showed a closer relationship between strain 3As(T) and Tm. intermedia (T) than between strain 3As(T) and Tm. perometabolis (T). These apparent contradictory conclusions were explained by differences in genome of these three bacteria, which are known to be highly flexible in Thiomonas spp. A novel species designation Thiomonas arsenitoxydans is proposed for strain 3As(T) (DSM 22701(T), CIP 110005(T)), which is nominated as the type strain of this species.
先前基于比较 16S rRNA 基因序列的研究结果表明，中度嗜酸、兼性化能自养、能够氧化 As(III)的 Thiomonas sp.（菌株 3As(T)）与 Tm. perometabolis DSM 18570(T) 和 Tm. intermedia DSM 18155(T) 密切相关。尽管它与 Tm. intermedia（T）有许多生理特征，但平均 DNA-DNA 杂交值（DDHV）为 47.2%，证实菌株 3As(T) 不是 Tm. intermedia（T）的菌株，尽管对于 Tm. perometabolis（之前确定的 DDHV 为 72%）的情况更为模糊。因此，对菌株 3As(T) 和 Tm. perometabolis（T）进行了比较生理和化学分类学研究，并对这三种细菌进行了多位点序列分析（MLSA）。脂肪酸图谱和有机底物利用的差异支持了菌株 3As(T) 和 Tm. perometabolis 是不同物种的观点，而 MLSA 显示菌株 3As(T) 与 Tm. intermedia（T）的关系比菌株 3As(T) 与 Tm. perometabolis（T）更密切。这些看似矛盾的结论可以通过这三种细菌基因组的差异来解释，众所周知，Thiomonas spp. 的基因组非常灵活。提议为菌株 3As(T)（DSM 22701(T)、CIP 110005(T)）创建一个新的物种名称 Thiomonas arsenitoxydans，该菌株被指定为该物种的模式菌株。
