Rab6 在人巨细胞病毒组装中的作用。
A role for the small GTPase Rab6 in assembly of human cytomegalovirus.
机构信息
Department of Pediatrics, School of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA.
出版信息
J Virol. 2011 May;85(10):5213-9. doi: 10.1128/JVI.02605-10. Epub 2011 Mar 16.
Abstract
In human-cytomegalovirus (HCMV)-infected cells, the localization of the viral protein pp150 to the virus assembly compartment (AC) is dependent on its direct interaction with the cellular protein Bicaudal D1 through a dynein- and microtubule-dependent mechanism. We found that the small GTPase Rab6 also interacts indirectly with pp150 through its interaction with Bicaudal D1. Inhibition of Rab6 activity in HCMV-infected cells interrupted the intracellular trafficking of pp150, significantly reducing infectious virus production without affecting the formation of the AC, arguing for an important function for this cellular GTPase in the intracellular localization of pp150 during virus assembly.
摘要
在人巨细胞病毒 (HCMV) 感染的细胞中，病毒蛋白 pp150 定位于病毒装配区 (AC) 依赖于其通过依赖于动力蛋白和微管的机制与细胞蛋白 Bicaudal D1 的直接相互作用。我们发现小 GTPase Rab6 也通过与 Bicaudal D1 的相互作用间接与 pp150 相互作用。在 HCMV 感染的细胞中抑制 Rab6 活性会中断 pp150 的细胞内运输，显著降低感染性病毒的产生，而不影响 AC 的形成，这表明这种细胞 GTPase 在病毒装配过程中 pp150 的细胞内定位中具有重要功能。
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