Department of Obstetrics and Gynecology, School of Medicine, Marmara University, Uskudar, Istanbul, Turkey.
Climacteric. 2011 Jun;14(3):384-91. doi: 10.3109/13697137.2010.550973. Epub 2011 Mar 17.
To determine the frequency of the vitamin D receptor (VDR) gene polymorphisms BsmI, ApaI, TaqI and FokI and their associations with bone mineral density (BMD) in postmenopausal Turkish women.
One hundred and thirty healthy postmenopausal women and 130 premenopausal healthy women acting as controls were included in the study. The BsmI, FokI, ApaI and TaqI polymorphisms in the VDR gene were studied by polymerase chain reaction-restriction fragment length polymorphism method. The BMD of the lumbar vertebrae and femur neck were measured by dual-energy X-ray absorptiometry. Comparisons between the groups were performed using the paired t-test and ANOVA. χ (2) or contingency tables were used to analyze qualitative results.
Genotypes BB, Bb and bb occurred in premenopausal women with frequencies of 16.92%, 50% and 33.08% and in postmenopausal women with frequencies of 16.92%, 56.15% and 26.92%, respectively. Genotypes FF, Ff, ff occurred in premenopausal women with frequencies of 47.69%, 42.31% and 10% and in postmenopausal women with frequencies of 50.77%, 42.31% and 6.92%, respectively. Genotypes AA, Aa, aa occurred in premenopausal women with frequencies of 23.85%, 56.15% and 20% and in postmenopausal women with frequencies of 26.15%, 46.15% and 27.70%, respectively. Genotypes TT, Tt and tt occurred in premenopausal women with frequencies of 37.69%, 45.38% and 16.92% and in postmenopausal women with frequencies of 39.23%, 45% and 15.38%, respectively. There was no difference in the frequencies of VDR gene polymorphisms between premenopausal and postmenopausal women. BMD measurements were not different between genotypes in premenopausal and postmenopausal women.
The VDR gene BsmI, FokI, ApaI and TaqI polymorphisms have no major influence on bone mineral density in our group of postmenopausal women.
确定维生素 D 受体（VDR）基因多态性 BsmI、ApaI、TaqI 和 FokI 的频率及其与绝经后土耳其女性骨密度（BMD）的关系。
本研究纳入 130 名健康绝经后女性和 130 名健康绝经前女性作为对照组。采用聚合酶链反应-限制性片段长度多态性方法研究 VDR 基因中的 BsmI、FokI、ApaI 和 TaqI 多态性。采用双能 X 线吸收法测量腰椎和股骨颈的 BMD。使用配对 t 检验和方差分析比较组间差异。使用 χ² 或列联表分析定性结果。
在绝经前女性中，BB、Bb 和 bb 基因型的出现频率分别为 16.92%、50%和 33.08%，在绝经后女性中分别为 16.92%、56.15%和 26.92%。在绝经前女性中，FF、Ff 和 ff 基因型的出现频率分别为 47.69%、42.31%和 10%，在绝经后女性中分别为 50.77%、42.31%和 6.92%。在绝经前女性中，AA、Aa 和 aa 基因型的出现频率分别为 23.85%、56.15%和 20%，在绝经后女性中分别为 26.15%、46.15%和 27.70%。在绝经前女性中，TT、Tt 和 tt 基因型的出现频率分别为 37.69%、45.38%和 16.92%，在绝经后女性中分别为 39.23%、45%和 15.38%。绝经前和绝经后女性 VDR 基因多态性的频率无差异。绝经前和绝经后女性中，各基因型的 BMD 测量值无差异。
在我们的绝经后女性群体中，VDR 基因 BsmI、FokI、ApaI 和 TaqI 多态性对骨矿物质密度没有主要影响。
