Samanta Swapan, Dey Pranab, Gupta Nalini, Mouleeswaran K S, Nijhawan Raje
Department of Pathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Diagn Cytopathol. 2011 Apr;39(4):242-4. doi: 10.1002/dc.21368.
The aim of this study was to explore the role of micronucleus (MN) scoring in distinguishing the smears of atypical squamous cell of undetermined significance (ASCUS) with reactive outcome versus ASCUS with cervical intraepithelial lesions (CIN) outcome. In this retrospective study, there were 53 cases of ASCUS diagnosed on cervical cytology smear and of which 30 cases showed reactive changes (group 1) and 23 cases showed CIN (group 2) on follow up histology. MN score of group 1 was compared with group 2 in conventional Papanicolaou's stained smear. The micronucleated cell per 1,000 epithelial cells in oil immersion magnification (100× objective) was counted by two observers and expressed as MN score. The data were compared in the two groups. The mean MN score was 2.8667 ± 2.20866 in group 1 and 8.3478 ± 6.44987 in group 2 cases. The Student's t-test showed significant difference of MN score in group 2 compared to group 1 (P < 0.0001). MN score may be helpful in identifying the true CIN cases that are mislabeled as ASCUS on cervical smear. In future, MN score can be used as an additional biomarker in cervical cancer screening.
本研究的目的是探讨微核（MN）评分在鉴别意义不明确的非典型鳞状细胞（ASCUS）涂片反应性结果与ASCUS伴宫颈上皮内病变（CIN）结果中的作用。在这项回顾性研究中，53例宫颈细胞学涂片诊断为ASCUS的病例，其中30例随访组织学显示为反应性改变（第1组），23例显示为CIN（第2组）。在传统巴氏染色涂片中比较第1组和第2组的MN评分。由两名观察者在油镜放大倍数（100×物镜）下计数每1000个上皮细胞中的微核细胞，并表示为MN评分。比较两组数据。第1组的平均MN评分为2.8667±2.20866，第2组病例为8.3478±6.44987。学生t检验显示第2组的MN评分与第1组相比有显著差异（P<0.0001）。MN评分可能有助于识别在宫颈涂片上被误标记为ASCUS的真正CIN病例。未来，MN评分可作为宫颈癌筛查中的一种额外生物标志物。
