新生大鼠视觉系统发育过程中Nogo-A和Nogo受体的表达
[Expression of Nogo-A and Nogo receptor in neonatal rats visual system during development].
作者信息
Huo Yan, Yuan Rong-di, Ye Jian, Yin Xiao-Lei, Zou Huan
机构信息
Department of Ophthalmology, Daping Hospital, the Third Military Medical University, Chongqing 400042, China.
出版信息
Zhonghua Yan Ke Za Zhi. 2011 Jan;47(1):54-8.
OBJECTIVE
To study the expression of Nogo-A and Nogo receptor (NgR) in the development of neonatal rats retinal and visual cortex and their effects on the development of central nervous system (CNS).
METHODS
Immunohistochemical staining method was used to detect the expression of Nogo-A and NgR in neonatal rats retinal and visual cortex at 1, 3, 7, 14, 28, 56 days after birth. One-way Anova was used to analyze the results of Western blot analysis of Nogo-A and NgR in neonatal rats visual cortex.
RESULTS
Nogo-A and NgR was found to express in neonatal rats retinal and visual cortex at the developing stage. In 1, 3, 7, 14, 28, 56 days, the IDV ratio of Nogo-A was 0.852 ± 0.026, 0.917 ± 0.024, 0.810 ± 0.028, 0.417 ± 0.053, 0.258 ± 0.029, 0.298 ± 0.054 respectively and the IDV ratio of NgR was 0.070 ± 0.014, 0.185 ± 0.035, 0.678 ± 0.046, 0.705 ± 0.021, 1.210 ± 0.057, 1.140 ± 0.0420 respectively by Western blot analysis. The expression of Nogo-A was decreased (F = 376.56, P = 0.000) and the expression of NgR was increased (F = 888.26, P = 0.000) during postnatal development.
CONCLUSION
Nogo-A and NgR is closely related to the CNS growth and development.
目的
研究新生大鼠视网膜和视皮层发育过程中Nogo-A及Nogo受体（NgR）的表达情况及其对中枢神经系统（CNS）发育的影响。
方法
采用免疫组织化学染色法检测出生后1、3、7、14、28、56天新生大鼠视网膜和视皮层中Nogo-A和NgR的表达。采用单因素方差分析对新生大鼠视皮层中Nogo-A和NgR的蛋白质免疫印迹分析结果进行分析。
结果
发现Nogo-A和NgR在新生大鼠视网膜和视皮层发育阶段有表达。蛋白质免疫印迹分析显示，在出生后1、3、7、14、28、56天，Nogo-A的相对光密度（IDV）比值分别为0.852±0.026、0.917±0.024、0.810±0.028、0.417±0.053、0.258±0.029、0.298±0.054，NgR的IDV比值分别为0.070±0.014、0.185±0.035、0.678±0.046、0.705±0.021、1.210±0.057、1.140±0.0420。出生后发育过程中，Nogo-A表达降低（F = 376.56，P = 0.000），NgR表达升高（F = 888.26，P = 0.000）。
结论
Nogo-A和NgR与中枢神经系统的生长发育密切相关。
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