[体外比较天然五倍子与中性五倍子对早期釉质龋损的再矿化增强作用]

[Comparison of the enhanced remineralization of initial enamel carious lesions treated with original and neutral Galla chinensis in vitro].

作者信息

Zhang Ling-Lin, Li Ji-Yao, Zhou Xue-Dong, Li Wei

机构信息

State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.

出版信息

Zhonghua Kou Qiang Yi Xue Za Zhi. 2011 Jan;46(1):7-11.

PMID:21418937

Abstract

OBJECTIVE

To compare the effect of original and neutral Galla chinensis in promoting the remineralization of initial enamel carious lesions in vitro and to investigate the influence of Galla chinensis with different pH on the promoting effect.

METHODS

Bovine sound enamel slabs were demineralized to produce initial carious lesion in vitro. Then the lesions were exposed to a pH-cycling regime for 12 days. Each daily cycle included 4 × 1 min application of one of four treatments: distilled and deionized water (DDW), aqueous solutions of NaF, acidic or neutral aqueous solutions of Galla chinensis extract (GCE). Before and after pH-cycling, the surface topography of the enamel slabs was observed by atomic force microscopy (AFM), and the integrated mineral loss and lesion depth of all the specimens were analysed by transverse microradiography.

RESULTS

AFM images revealed the surface topographical changes of GCE-treated enamel. The percentage change of integrated mineral loss (ΔIML%) of the samples of NaF group, DDW group, pH 3.8 GCE group and pH 7.0 GCE group was (-38 ± 14)%, (+43 ± 7)%, (-10 ± 4)% and (-11 ± 4)% respectively. The percentage of lesion depth (ΔLD%) of the samples of NaF group, DDW group, pH 3.8 GCE group and pH 7.0 GCE group was (-27.79 ± 3.51)%, (+21.13 ± 2.83)%, (-8.43 ± 3.32)% and (-9.20 ± 3.89)% respectively. There was no significant difference in ΔIML% and ΔLD% between pH 3.8 and pH 7.0 GCE-treated enamel.

CONCLUSIONS

There is no significant difference in enhancement of remineralization of initial enamel carious lesions between the original and neutral Galla chinensis. Different pH Galla chinensis does not have obvious influence on remineralization. It is unnecessary to regulate the pH value of queous solution of Galla chinensis extract which acts as a anti-caries agent.

摘要

目的

比较生五倍子和中性五倍子促进早期釉质龋损体外再矿化的效果，并探讨不同pH值的五倍子对其促进作用的影响。

方法

将牛的健康釉质块在体外脱矿以产生早期龋损。然后将龋损置于pH循环环境中12天。每个循环包括4次1分钟的四种处理之一：蒸馏水和去离子水（DDW）、NaF水溶液、酸性或中性五倍子提取物（GCE）水溶液。在pH循环前后，通过原子力显微镜（AFM）观察釉质块的表面形貌，并通过横向显微放射摄影分析所有标本的累积矿物质流失和龋损深度。

结果

AFM图像显示了GCE处理后釉质的表面形貌变化。NaF组、DDW组、pH 3.8 GCE组和pH 7.0 GCE组样本的累积矿物质流失百分比变化（ΔIML%）分别为（-38±14）%、（+43±7）%、（-10±4）%和（-11±4）%。NaF组、DDW组、pH 3.8 GCE组和pH 7.0 GCE组样本的龋损深度百分比（ΔLD%）分别为（-27.79±3.51）%、（+21.13±2.83）%、（-8.43±3.32）%和（-9.20±3.89）%。pH 3.8和pH 7.0的GCE处理的釉质之间的ΔIML%和ΔLD%没有显著差异。