口腔鳞状细胞癌中TP53、D9S1747、D9S162和RPS6的分子分析

[Molecular analysis of TP53, D9S1747, D9S162 and RPS6 in oral squamous cell carcinoma].

作者信息

Wang Xin-Hong, Zhang Ying-Ying, Chen Xin-Ming, Sun Zhi-Jun

机构信息

Department of Pathology, School of Stomatology, Wuhan University, Wuhan 430079, China.

出版信息

Zhonghua Kou Qiang Yi Xue Za Zhi. 2011 Jan;46(1):42-6.

PMID:21418946

Abstract

OBJECTIVE

To investigate the correlation between the frequency of molecular abnormalities of 4 loci at chromosomal 9p21 (D9S1747, D9S162, RPS6) and 17p13 (TP53) and the clinical characteristics and prognosis.

METHODS

The oral squamous cell carcinoma (OSCC) lesions in 71 patients were manually microdessected. Genomic DNA from these lesions and normal lymphnode tissu or peripheral blood of the same patients were extracted using the Watson's tissue kit. The loss of heterozygosity (LOH) and microsatellite instability (MI) of 17p13 and 9p21 were analyzed by PCR-page electrophoresis after DNA extraction.

RESULTS

LOH and MI were detected in the OSCC of 48 patients (68%). The LOH and MI frequency at chromosomes 17p13 and 9P21 were 56% (35/63) and 59% (40/68) respectively. The LOH and MI frequency at 9p21 was significantly associated with WHO grading (P < 0.01) and lymphonode metastasis (P < 0.01). The LOH and MI frequency at 17p13 was significantly associated with clinical stage (P < 0.05). TP53 genetic aberration and 9p21 genetic aberration were significant prognostic factors for OSCC. The prognosis was poor in the LOH and MI positive group of chromosome 17p13 and 9p21. The frequency of LOH and MI at TP53 was the only independent factor for overall survival (P < 0.05).

CONCLUSIONS

The LOH and MI of 17p13 and 9p21 were related to clinical stage and lymphonode metastasis. LOH of TP53 was an independent prognostic factor for OSCC.

摘要

目的

研究9号染色体短臂21区（D9S1747、D9S162、RPS6）和17号染色体短臂13区（TP53）4个位点分子异常频率与临床特征及预后的相关性。

方法

对71例患者的口腔鳞状细胞癌（OSCC）病变进行手工显微切割。使用沃森组织试剂盒从这些病变组织以及同一患者的正常淋巴结组织或外周血中提取基因组DNA。DNA提取后，通过聚合酶链反应-聚丙烯酰胺凝胶电泳分析17号染色体短臂13区和9号染色体短臂21区的杂合性缺失（LOH）和微卫星不稳定性（MI）。

结果

48例（68%）OSCC患者检测到LOH和MI。17号染色体短臂13区和9号染色体短臂21区的LOH和MI频率分别为56%（35/63）和59%（40/68）。9号染色体短臂21区的LOH和MI频率与世界卫生组织分级（P<0.01）和淋巴结转移（P<0.01）显著相关。17号染色体短臂13区的LOH和MI频率与临床分期显著相关（P<0.05）。TP53基因畸变和9号染色体短臂21区基因畸变是OSCC的重要预后因素。17号染色体短臂13区和9号染色体短臂21区LOH和MI阳性组预后较差。TP53的LOH和MI频率是总生存的唯一独立因素（P<0.05）。