Han Xue, Liu Hong-Chen, Wang Dong-Sheng, Li Shu-Jun, Yang Rui
Dental Institute, General Hospital of PLA, Beijing 100853, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2011 Jan;46(1):50-3.
To observe the efficacy of micro-arc oxidation and alkali-heat treatment (MAH) on Ti-24Nb-4Zr-8Sn (Ti2448).
Disks (diameter of 14.5 mm, thickness of 1 mm) and cylinders (diameter of 3 mm, height of 10 mm) were fabricated from Ti2448 alloy. Samples were divided into three groups: polished (Ti2448), micro-arc oxidation(MAO-Ti2448), micro-arc oxidation and alkali-heat treatment (MAH-Ti2448). MC3T3-E1 osteoblastic cells were cultured on the disks and cell morphology was observed with scanning electron microscopy (SEM) aftre 3 days. The cylinder samples were implanted in the tibia of dogs and implant-bone interface was observed with SEM after 3 months.
A rough and porous structure was shown in both MAO and MAH group. The MC3T3-E1 cells on the MAH-Ti2448 discs spread fully in intimate contact with the underlying coarse surface through active cytoskeletal extentions. Osseointegration was formed in the implant-bone interface in MAH samples.
MAH treatment can provide a more advantageous Ti2448 surface to osteoblastic cells than MAO treatment does, and the former can improve the implant-bone integration.
观察微弧氧化和碱热处理（MAH）对Ti-24Nb-4Zr-8Sn（Ti2448）的效果。
用Ti2448合金制作圆盘（直径14.5mm，厚度1mm）和圆柱（直径3mm，高度10mm）。将样品分为三组：抛光组（Ti2448）、微弧氧化组（MAO-Ti2448）、微弧氧化和碱热处理组（MAH-Ti2448）。将MC3T3-E1成骨细胞接种于圆盘上，3天后用扫描电子显微镜（SEM）观察细胞形态。将圆柱样品植入犬胫骨，3个月后用SEM观察植入物与骨的界面。
微弧氧化组和微弧氧化和碱热处理组均呈现出粗糙多孔的结构。MAH-Ti2448圆盘上的MC3T3-E1细胞通过活跃的细胞骨架延伸充分铺展，与下方粗糙表面紧密接触。MAH样品的植入物与骨界面形成了骨整合。
与微弧氧化处理相比，碱热处理能为成骨细胞提供更有利的Ti2448表面，且前者可改善植入物与骨的整合。
