远志根提取物对苯并[a]芘诱导的小鼠肺癌的抗癌潜力

Anticancer potentials of root extract of Polygala senega against benzo[a]pyrene-induced lung cancer in mice.

作者信息

Paul Saili, Bhattacharyya Soumya Sundar, Samaddar Asmita, Boujedaini Naoual, Khuda-Bukhsh Anisur Rahman

机构信息

Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India.

出版信息

Zhong Xi Yi Jie He Xue Bao. 2011 Mar;9(3):320-7. doi: 10.3736/jcim20110314.

DOI:10.3736/jcim20110314

PMID:21419086

Abstract

OBJECTIVE

To evaluate anticancer potentials of Polygala senega on lung cancer induced by benzo[a]pyrene (B[a]P) in mice.

METHODS

Swiss albino mice were divided into five groups with each containing six animals. Group 1 served as control, and the animals received olive oil as vehicle. Group 2 animals were treated with B[a]P (50 mg/kg body weight dissolved in olive oil) orally twice a week for four consecutive weeks. Group 3 animals were fed B[a]P as in group 2 and 48% alcohol (since the vehicle of the remedy was alcohol). Group 4 animals were B[a]P-intoxicated mice (as in group 2) which were additionally fed ethanolic extract of Polygala senega (EEPS) daily for 16 weeks. EEPS treatment started after the first dose of B[a]P. Group 5 animals were treated with EEPS alone for 16 weeks to test cytotoxicity of EEPS if any. Mice were sacrificed after 16 weeks and the following parameters were assessed: the anti-oxidant activity measured by 2,2-diphenyl-1-picrylhydrazyl free radical assay, tumor incidence, lung weight and body weight, DNA damage evaluation by comet assay and enzyme-linked immunosorbent assay (ELISA); toxicity biomarkers like catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, lipid peroxidation (LPO) and total thiol content were also detected.

RESULTS

Treatment with EEPS increased the final body weight and significantly decreased the lung weight in group 4 mice (P<0.01) compared with group 3 mice. Comet assay showed that EEPS-treated mice in group 4 presented a decrease of DNA damage significantly (P<0.01) in lung tissues. There was a significant increase observed in the level of p53 in group 4 as compared with group 3 (P<0.01) detected by ELISA. A highly significant increase in tissue LPO with concomitant decrease in the activity of anti-oxidants was observed in group 2 and group 3 mice (P<0.05) compared with the control mice. These adverse changes were reversed significantly in group 4 mice (P<0.01).

CONCLUSION

Chemopreventive potentials of Polygala senega against chemically induced lung cancer in mice are confirmed.

摘要

目的

评估美远志对苯并[a]芘（B[a]P）诱导的小鼠肺癌的抗癌潜力。

方法

将瑞士白化小鼠分为五组，每组六只动物。第1组作为对照组，动物接受橄榄油作为赋形剂。第2组动物每周口服两次B[a]P（50毫克/千克体重，溶于橄榄油），连续四周。第3组动物如第2组一样接受B[a]P处理，并给予48%乙醇（因为药物的赋形剂是乙醇）。第4组动物为B[a]P中毒小鼠（如第2组），另外每天给予美远志乙醇提取物（EEPS），持续16周。EEPS处理在首次给予B[a]P后开始。第5组动物单独给予EEPS处理16周，以测试EEPS是否有细胞毒性。16周后处死小鼠，评估以下参数：通过2,2-二苯基-1-苦基肼自由基测定法测量的抗氧化活性、肿瘤发生率、肺重量和体重、通过彗星试验和酶联免疫吸附测定（ELISA）评估的DNA损伤；还检测了过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、脂质过氧化（LPO）和总巯基含量等毒性生物标志物。

结果

与第3组小鼠相比，第4组小鼠经EEPS处理后最终体重增加，肺重量显著降低（P<0.01）。彗星试验表明，第4组经EEPS处理的小鼠肺组织中的DNA损伤显著减少（P<0.01）。通过ELISA检测，与第3组相比，第4组中p53水平显著升高（P<0.01）。与对照小鼠相比，第2组和第3组小鼠的组织LPO显著增加，同时抗氧化剂活性降低（P<0.05）。这些不利变化在第4组小鼠中得到显著逆转（P<0.01）。