Pushkarev V M, Starenki D V, Saenko V O, Tronko M D, Yamashita S
State Institution V.P. Komisarenko Institute of Endocrinology and Metabolism, AMS of Ukraine, Kyiv 04114, Ukraine.
Exp Oncol. 2011 Mar;33(1):24-7.
To study the effects of Paclitaxel (Ptx), γ-irradiation (IR) and their combination on the growth of xenografted tumors derived from undifferentiated thyroid cancer cells.
Experiments were performed in nude mice with tumors developing from implanted undifferentiated thyroid carcinoma cells (FRO). Animals were treated with Ptx i.p. and exposed locally to a single dose of 5 Gy of IR. Apoptosis in situ was detected using ApopTag Peroxidase Kit.
In the in vivo experiments, IR significantly inhibited but did not abrogate tumor growth. Ptx effect was stronger, and the combination therapy with Ptx and IR led to the decrease of tumor volume to 0-0.3% of the control (P < 0.01). The systemic administration of Ptx to the animals with advanced tumors resulted in a profound tumor growth suppression and in apoptosis in tumor tissues in time-dependent manner.
The combination of Ptx and IR is a promising strategy for further preclinical and clinical trials aimed at the development of new therapeutic approaches to the treatment of undifferentiated thyroid cancer.
研究紫杉醇（Ptx）、γ射线辐射（IR）及其联合应用对源自未分化甲状腺癌细胞的异种移植肿瘤生长的影响。
在接种未分化甲状腺癌细胞（FRO）形成肿瘤的裸鼠中进行实验。动物接受腹腔注射Ptx，并局部接受单次5 Gy的IR照射。使用ApopTag过氧化物酶试剂盒检测原位凋亡。
在体内实验中，IR显著抑制但未消除肿瘤生长。Ptx的作用更强，Ptx与IR联合治疗导致肿瘤体积减小至对照组的0 - 0.3%（P < 0.01）。对晚期肿瘤动物全身给药Ptx导致肿瘤生长受到显著抑制，并使肿瘤组织中的细胞凋亡呈时间依赖性。
Ptx与IR联合应用是一种有前景的策略，可用于进一步的临床前和临床试验，旨在开发治疗未分化甲状腺癌的新治疗方法。
