DNA修复基因XRCC1和ERCC4的多态性与波兰人群中吸烟和饮酒相关的喉癌无关。
Polymorphisms of the DNA repair genes XRCC1 and ERCC4 are not associated with smoking- and drinking-dependent larynx cancer in a Polish population.
作者信息
Krupa R, Kasznicki J, Gajęcka M, Rydzanicz M, Kiwerska K, Kaczmarczyk D, Olszewski J, Szyfter K, Blasiak J, Morawiec-Sztandera A
机构信息
Department of Molecular Genetics, University of Lodz, Lodz 90-236, Poland.
出版信息
Exp Oncol. 2011 Mar;33(1):55-6.
BACKGROUND
Tobacco smoking and alcohol drinking generate oxidative DNA damage and may contribute to larynx carcinogenesis. The X-ray repair cross complementing 1 (XRCC1) and excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4(XPF)) genes are important components of DNA excision repair systems, which repair DNA damage induced by various factors, including tobacco smoking and alcohol.
AIM
To investigate the association between the genotypes of the XRCC1-Arg399Gln (rs25487) and ERCC4-Arg415Gln (rs1800067) polymorphisms and smoking- and drinking-related larynx cancer in a Polish population.
METHODS
The polymorphisms were determined by PCR-RFLP method in 253 patients with squamous cell carcinoma of the larynx and 253 sex- and age-matched controls.
RESULTS
We did not find any association between the investigated polymorphisms and larynx carcinoma, dependent on either smoking or drinking status. No association was found between these polymorphisms and larynx cancer grade, stage or age at diagnosis.
CONCLUSIONS
The results indicated that Arg399Gln polymorphism of XRCC1 gene and Arg415Gln polymorphism of ERCC4 gene may not be associated with smoking- and drinking-related larynx cancer in Polish population.
背景
吸烟和饮酒会导致氧化性DNA损伤，可能促使喉癌发生。X射线修复交叉互补蛋白1（XRCC1）和切除修复交叉互补蛋白啮齿动物修复缺陷互补组4（ERCC4(XPF)）基因是DNA切除修复系统的重要组成部分，该系统可修复由包括吸烟和饮酒在内的各种因素诱导的DNA损伤。
目的
在波兰人群中研究XRCC1基因的Arg399Gln（rs25487）和ERCC4基因的Arg415Gln（rs1800067）多态性基因型与吸烟和饮酒相关喉癌之间的关联。
方法
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对253例喉鳞状细胞癌患者和253例性别及年龄匹配的对照者进行多态性检测。
结果
无论吸烟或饮酒状况如何，我们均未发现所研究的多态性与喉癌之间存在任何关联。这些多态性与喉癌分级、分期或诊断时的年龄之间也未发现关联。
结论
结果表明，在波兰人群中，XRCC1基因的Arg399Gln多态性和ERCC4基因的Arg415Gln多态性可能与吸烟和饮酒相关喉癌无关。
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