单纯疱疹病毒 1 衣壳蛋白 VP22 的宿主细胞靶标。

Host cell targets of tegument protein VP22 of herpes simplex virus 1.

机构信息

State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.

出版信息

Arch Virol. 2011 Jun;156(6):1079-84. doi: 10.1007/s00705-011-0960-9. Epub 2011 Mar 22.

Abstract

In the present study, seven novel host cell factors associated with VP22 were identified from the human leukocyte cDNA library using a yeast two-hybrid high-throughput screening system. To confirm some of the interactions, VP22 and its homologues and two candidate targets were tagged with enhanced cyan or yellow fluorescent protein. While RING finger protein 10 (RNF10), WD repeat-containing protein 42A (WDR42A) or VP22 alone showed distinct subcellular localization patterns, RNF10 and WDR42A were relocated when co-expressed with VP22 or its homologues. Thus, these potential host cell factors of VP22 might expand the list of the host targets of VP22.

摘要

在本研究中，我们利用酵母双杂交高通量筛选系统从人白细胞 cDNA 文库中鉴定出与 VP22 相关的七个新的宿主细胞因子。为了确认部分相互作用，我们将 VP22 及其同源物和两个候选靶标标记为增强型青色或黄色荧光蛋白。虽然 RING 指蛋白 10（RNF10）、WD 重复蛋白 42A（WDR42A）或 VP22 单独显示出不同的亚细胞定位模式，但当与 VP22 或其同源物共表达时，RNF10 和 WDR42A 会被重新定位。因此，这些 VP22 的潜在宿主细胞因子可能会扩展 VP22 的宿主靶标列表。