用于研究分子病毒学的高通量酵母双杂交技术

High-Throughput Yeast Two-Hybrid Technology for Investigating Molecular Virology.

作者信息

Xia Sisi, Wang Jialin, Huo Xiaoping, Zheng Chunfu, Li Hongjian, Chen Jun

机构信息

Department of Biological Engineering, Wuhan Polytechnic University, Wuhan, China.

State Key Laboratory of Bioactive Molecules and Druggability Assessment, Jinan University, Guangzhou, China.

出版信息

Methods Mol Biol. 2025;2940:121-132. doi: 10.1007/978-1-0716-4615-1_11.

DOI:10.1007/978-1-0716-4615-1_11

PMID:40515906

Abstract

High-throughput yeast two-hybrid (YTH) screening systems are essential technologies for understanding the viral life cycle, pathogenesis, and development of antiviral therapies. They are commonly used in molecular virology to identify and analyze the complex molecular interactions between viruses and host cells. This chapter details the relevant information and experimental procedures for systematically screening and identifying interactions between viral and host proteins via high-throughput YTH technology.

摘要

高通量酵母双杂交（YTH）筛选系统是了解病毒生命周期、发病机制和抗病毒疗法开发的关键技术。它们在分子病毒学中常用于识别和分析病毒与宿主细胞之间复杂的分子相互作用。本章详细介绍了通过高通量YTH技术系统筛选和鉴定病毒与宿主蛋白之间相互作用的相关信息和实验步骤。