Kowalewski R, Miltzow M
Institut für Anatomie des Bereichs Medizin der Ernst-Moritz-Arndt-Universität Greifswald, DDR.
Anat Anz. 1990;170(3-4):205-11.
Muscle biopsies of the mandible adductors of the Wistar rat (Rattus norvegicus Berkenhout) have been analyzed enzyme-histochemically for the investigation of the postnatal development (42.-126. d post partum) of their muscle fibers with special regards to the fiber types. The following methods have been used in this investigation: myofibrillar adenosine triphosphatase (ATP-ase) with different pre-incubations, Sudan black B (triglycerides), periodic acid-Schiff reagent (PAS) (glycogen), Gomori modified Trichrome staining. Quantitative analysis of muscle fibre-type composition and muscle fibre size was done from different regions of muscle sections. Estimation of the fiber size was carried out by measuring the minimum diameter of each type of muscle fibre.
对Wistar大鼠（褐家鼠）下颌内收肌进行了肌肉活检，采用酶组织化学方法研究其肌纤维在出生后发育阶段（产后42 - 126天）的情况，特别关注纤维类型。本研究使用了以下方法：不同预孵育条件下的肌原纤维三磷酸腺苷酶（ATP酶）、苏丹黑B（甘油三酯）、过碘酸希夫试剂（PAS）（糖原）、Gomori改良三色染色法。从肌肉切片的不同区域对肌纤维类型组成和肌纤维大小进行定量分析。通过测量每种肌纤维的最小直径来估计纤维大小。
