一种基于 2,7-咔唑的双阳离子盐，用于荧光检测核酸以及核仁与细胞质中 RNA 的双光子荧光成像。
A 2,7-carbazole-based dicationic salt for fluorescence detection of nucleic acids and two-photon fluorescence imaging of RNA in nucleoli and cytoplasm.
机构信息
State Key Laboratory of Crystal Materials, Shandong University, Jinan, P R China.
出版信息
Org Biomol Chem. 2011 May 21;9(10):3615-8. doi: 10.1039/c1ob05123g. Epub 2011 Mar 23.
PMID:21431187
Abstract
A new carbazole-derived dicationic compound, namely 2,7-bis(1-hydroxyethyl-4-vinylpyridinium iodine)-N-ethylcarbazole (2,7-9E-BHVC), with a large two-photon action absorption cross section in nucleic acids has been obtained. Moreover, it possesses the potential of imaging RNA in nucleoli and cytoplasm in two-photon fluorescence microscopy and exhibits good counterstain compatibility with the commercial fluorescent nucleic dye DAPI.
摘要
一种新的咔唑衍生的双阳离子化合物，即 2,7-双（1-羟乙基-4-乙烯基吡啶碘）-N-乙基咔唑（2,7-9E-BHVC），具有在核酸中具有大的双光子作用吸收截面。此外，它具有在双光子荧光显微镜中对核仁及细胞质中 RNA 成像的潜力，并且与商业荧光核酸染料 DAPI 具有良好的复染相容性。
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