绒毛膜绒毛中β-N-乙酰己糖胺酶中间形式的鉴定。

Identification of an intermediate form of beta-N-acetylhexosaminidase in chorionic villi.

作者信息

Pieretti M, Beccari T, Cosmi E V, Stirling J L, Orlacchio A

机构信息

Istituto Ginecologia ed Ostetricia, University of Perugia, Italy.

出版信息

Clin Chim Acta. 1990 Apr 30;188(2):109-17. doi: 10.1016/0009-8981(90)90155-l.

DOI:10.1016/0009-8981(90)90155-l

PMID:2143122

Abstract

A minor form of beta-N-acetylhexosaminidase has been found in chorionic villi, in addition to the major forms A and B. This form does not hydrolyze the 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulpha te substrate, is thermostable, has a higher mol mass (120,000) than A and B (100,000) and on analytical isoelectric focusing, it shows a microheterogeneity with values ranging between 6.3 and 7.0. For these characteristics, it resembles beta-N-Acetylhexosaminidase P from pregnancy serum, from which is chromatographically indistinguishable.

摘要

除了主要形式A和B外，在绒毛膜绒毛中还发现了一种次要形式的β-N-乙酰己糖胺酶。这种形式不水解4-甲基伞形酮基-2-乙酰氨基-2-脱氧-β-D-吡喃葡萄糖苷-6-硫酸盐底物，具有热稳定性，分子量（120,000）高于A和B（100,000），在分析等电聚焦时，它显示出微异质性，值在6.3至7.0之间。基于这些特征，它类似于妊娠血清中的β-N-乙酰己糖胺酶P，在色谱上无法区分。