用荧光底物4-甲基伞形酮基-β-N-乙酰氨基葡萄糖6-硫酸盐鉴定小鼠组织中的β-N-乙酰己糖胺酶A
Identification of beta-N-acetylhexosaminidase A in mouse tissues with the fluorigenic substrate 4-methylumbelliferyl-beta-N-acetylglucosamine 6-sulphate.
作者信息
Beccari T, Orlacchio A, Stirling J L
机构信息
Istituto di Chimica Biologica, Facoltà di Scienza MM.FF.NN, Università di Perugia, Italy.
出版信息
Biochem J. 1988 Jun 1;252(2):617-20. doi: 10.1042/bj2520617.
Abstract
beta-N-Acetylhexosaminidase from mouse tissue was separated into its constituent isoenzymes on DEAE-cellulose and its activity was monitored with 4-methylumbelliferyl-beta-N-acetylglucosamine and 4-methylumbelliferyl-beta-N-acetylglucosamine 6-sulphate. Forms corresponding to the human isoenzymes A (acidic), B (basic) and an 'intermediate' form were present in mouse liver and spleen, whereas in kidney the B and 'intermediate' forms predominated, with A present only as a minor component. In brain the 'intermediate', A and C activities were detected. Testis had predominantly A activity, whereas epididymis, the tissue with the highest specific activity of beta-N-acetylhexosaminidase, had an abundance of the 'intermediate' form, but was almost entirely lacking in the A form.
摘要
从小鼠组织中提取的β-N-乙酰己糖胺酶在二乙氨基乙基纤维素上被分离成其组成同工酶，并用4-甲基伞形酮基-β-N-乙酰氨基葡萄糖和4-甲基伞形酮基-β-N-乙酰氨基葡萄糖6-硫酸盐监测其活性。在小鼠肝脏和脾脏中存在与人同工酶A（酸性）、B（碱性）和“中间”形式相对应的形式，而在肾脏中，B和“中间”形式占主导，A仅作为次要成分存在。在大脑中检测到“中间”、A和C活性。睾丸主要具有A活性，而附睾是β-N-乙酰己糖胺酶比活性最高的组织，富含“中间”形式，但几乎完全缺乏A形式。
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