Krause Ulf, Seckinger Anja, Gregory Carl A
Institute for Regenerative Medicine at Scott and White Hospital, Texas A and M Health Science Center, 5701 Airport Rd Module C, Temple, 76502, TX, USA.
Methods Mol Biol. 2011;698:215-30. doi: 10.1007/978-1-60761-999-4_17.
One of the most noteworthy characteristics of mesenchymal stem cells (MSCs) is their ability to differentiate into osteoblasts in vitro and in vivo. In vitro, this is easily achieved by culturing in the appropriate induction medium. It is because of the reliability and ease of this process that osteogenic differentiation has become a popular assay for the demonstration of MSC plasticity. Although the conditions required for inducing osteogenic differentiation by MSCs typically do not vary particularly between investigators, many methods are employed to measure the extent of differentiation. These methods include, but are not limited to, reverse transcriptase PCR (RT-PCR) for detection of osteogenic transcripts, enzyme linked immunosorbent assay (ELISA) for secreted protein markers, colorimetric assays for osteogenic enzymes, and direct staining of matrix components. This chapter reviews the protocols most commonly utilized for the evaluation of osteogenic differentiation for cultured MSCs.
间充质干细胞（MSCs）最显著的特征之一是其在体外和体内分化为成骨细胞的能力。在体外，通过在适当的诱导培养基中培养很容易实现这一点。正是由于这个过程的可靠性和简便性，成骨分化已成为用于证明MSC可塑性的一种常用检测方法。尽管不同研究者诱导MSCs进行成骨分化所需的条件通常差异不大，但仍有许多方法用于测量分化程度。这些方法包括但不限于：用于检测成骨转录本的逆转录聚合酶链反应（RT-PCR）、用于分泌蛋白标志物的酶联免疫吸附测定（ELISA）、用于成骨酶的比色测定以及基质成分的直接染色。本章回顾了培养的MSCs成骨分化评估中最常用的方案。
