Mercer Bruce R, Rayeroux Kathleen C
Victorian Cancer Cytogenetics Service, St. Vincent's Hospital Melbourne, Fitzroy, VIC, Australia.
Methods Mol Biol. 2011;730:159-71. doi: 10.1007/978-1-61779-074-4_12.
The low proliferation rate of myeloma cells in vitro can result in a normal cytogenetic karyotype with the abnormal cell population not being detected. Because plasma cell myeloma is a patchy disease, conventional FISH is also hampered by normal cell contamination. Identification of plasma cells by cytoplasmic immunoglobulin staining in combination with FISH (cIg FISH) can ensure that only the cells of interest are analyzed, and thus the results obtained are a more accurate reflection of the plasma cell population karyotype. Current literature suggests that probes for t(4;14), t(14;16), and del(17)(p13) should be used in routine diagnostic testing; however, this technique can be used for any probes of interest. In this chapter, we present the techniques and methods used in our laboratory for the detection of abnormalities in plasma cells by cIg staining in conjunction with FISH.
骨髓瘤细胞在体外的低增殖率可能导致细胞遗传学核型正常，异常细胞群体未被检测到。由于浆细胞骨髓瘤是一种局灶性疾病，传统的荧光原位杂交（FISH）也会受到正常细胞污染的影响。通过细胞质免疫球蛋白染色结合FISH（cIg FISH）来鉴定浆细胞，可以确保只分析感兴趣的细胞，因此获得的结果能更准确地反映浆细胞群体的核型。目前的文献表明，在常规诊断检测中应使用针对t(4;14)、t(14;16)和del(17)(p13)的探针；然而，该技术可用于任何感兴趣的探针。在本章中，我们介绍了我们实验室用于通过cIg染色结合FISH检测浆细胞异常的技术和方法。
