与RNA诱导沉默复合体相关的腺病毒小RNA的特征分析

Characterization of RISC-associated adenoviral small RNAs.

作者信息

Xu Ning, Akusjärvi Göran

机构信息

Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center (BMC), Uppsala, Sweden.

出版信息

Methods Mol Biol. 2011;721:183-98. doi: 10.1007/978-1-61779-037-9_11.

DOI:10.1007/978-1-61779-037-9_11

PMID:21431686

Abstract

RNA interference (RNAi) plays novel roles in both host antiviral defense and viral replication. It has been shown that some viruses can exploit the RNAi machinery for their own benefit by encoding for their own viral small RNAs. Here we present a collection of methods to study adenoviral small RNAs, specifically a method for immunopurification of RNA-induced silencing complex (RISC) and a biochemical assay for the activity of purified RISC associated with adenoviral small RNAs.

摘要

RNA干扰（RNAi）在宿主抗病毒防御和病毒复制中都发挥着新的作用。已经表明，一些病毒可以通过编码自身的病毒小RNA来利用RNAi机制为自身谋利。在此，我们介绍了一系列研究腺病毒小RNA的方法，特别是一种用于免疫纯化RNA诱导沉默复合体（RISC）的方法以及一种用于检测与腺病毒小RNA相关的纯化RISC活性的生化分析方法。