源自腺病毒VAI RNA的小RNA的序列特异性干扰。

Sequence-specific interference by small RNAs derived from adenovirus VAI RNA.

作者信息

Sano Masayuki, Kato Yoshio, Taira Kazunari

机构信息

Gene Function Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Tsukuba Science City 305-8562, Japan.

出版信息

FEBS Lett. 2006 Mar 6;580(6):1553-64. doi: 10.1016/j.febslet.2006.01.085. Epub 2006 Feb 3.

DOI:10.1016/j.febslet.2006.01.085

PMID:16472808

Abstract

A virus-associated RNA (VAI) of adenoviruses is a cytoplasmic non-coding RNA and it plays an important role for viral replication in infected cells. VAI RNA transcripts, produced by RNA polymerase III (pol III), form tightly structured stems, which confer resistance to cellular defense systems. We demonstrate here that small RNAs of approximately 22 nucleotides are produced from a terminal stem region but not from an apical stem of VAI RNA. We determined the processing sites of VAI RNA by S1 nuclease mapping and further confirmed that the processed small RNA can act as small interfering RNAs (siRNAs) or as microRNAs (miRNAs) in transient transfection assays and during viral infection. Our data demonstrate that non-coding RNAs synthesized by pol III can be substrates for Dicer, and diced small RNAs might regulate cellular phenomena as siRNAs and miRNAs.

摘要

腺病毒的病毒相关RNA（VAI）是一种细胞质非编码RNA，在受感染细胞的病毒复制中发挥重要作用。由RNA聚合酶III（pol III）产生的VAI RNA转录本形成紧密结构的茎，赋予对细胞防御系统的抗性。我们在此证明，约22个核苷酸的小RNA是从VAI RNA的末端茎区域而非顶端茎产生的。我们通过S1核酸酶图谱分析确定了VAI RNA的加工位点，并进一步证实加工后的小RNA在瞬时转染实验和病毒感染期间可作为小干扰RNA（siRNA）或微小RNA（miRNA）发挥作用。我们的数据表明，由pol III合成的非编码RNA可以作为Dicer的底物，切割后的小RNA可能作为siRNA和miRNA调节细胞现象。