成年果蝇对双链RNA的摄取

dsRNA uptake in adult Drosophila.

作者信息

Obadia Benjamin, Saleh Maria-Carla

机构信息

CNRS URA 3015, Institut Pasteur, Viruses and RNAi group, Paris, France.

出版信息

Methods Mol Biol. 2011;721:253-63. doi: 10.1007/978-1-61779-037-9_16.

DOI:10.1007/978-1-61779-037-9_16

PMID:21431691

Abstract

RNA interference (RNAi) is a conserved sequence-specific gene silencing mechanism that is induced by double-stranded RNA (dsRNA). The development of methods that allow internalization of dsRNA and concomitant silencing of the desired gene has not stopped since the first demonstration of RNAi in Caenorhabditis elegans. In this chapter, we describe how to introduce exogenous dsRNA into adult Drosophila in order to interfere with endogenous or viral gene expression.

摘要

RNA干扰（RNAi）是一种由双链RNA（dsRNA）诱导的保守的序列特异性基因沉默机制。自从在秀丽隐杆线虫中首次证明RNAi以来，能够使dsRNA内化并同时沉默所需基因的方法的开发就从未停止过。在本章中，我们将描述如何将外源性dsRNA导入成年果蝇体内，以干扰内源性或病毒基因的表达。