Sweetman Dylan
School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, UK.
Methods Mol Biol. 2011;732:1-8. doi: 10.1007/978-1-61779-083-6_1.
The detection of microRNAs (miRNAs) in situ presents several technical challenges. Although protocols for mRNA detection by in situ hybridization are well established, the small size of miRNAs makes their localization problematic. To overcome this, digoxygenin-labeled locked nucleic acid oligos have been used. These bind strongly and specifically to miR targets and make the identification of the precise spatiotemporal expression of these molecules possible.
原位检测微小RNA（miRNA）存在若干技术挑战。尽管通过原位杂交检测mRNA的方案已经很成熟，但miRNA的小尺寸使其定位存在问题。为克服这一问题，已使用地高辛标记的锁核酸寡核苷酸。这些寡核苷酸能与miRNA靶标强烈且特异性地结合，从而使确定这些分子精确的时空表达成为可能。
