使用锁核酸（LNA）修饰的寡核苷酸探针原位检测动物胚胎中的微小RNA（miRNA）

In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes.

作者信息

Kloosterman Wigard P, Wienholds Erno, de Bruijn Ewart, Kauppinen Sakari, Plasterk Ronald H A

机构信息

Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.

出版信息

Nat Methods. 2006 Jan;3(1):27-9. doi: 10.1038/nmeth843.

DOI:10.1038/nmeth843

PMID:16369549

Abstract

MicroRNAs (miRNAs) are 20-23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development. Here we performed a detailed analysis of conditions for in situ detection of miRNAs in the zebrafish embryo using locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo.

摘要

微小RNA（miRNA）是20 - 23个核苷酸（nt）的RNA分子，可在转录后调节基因表达。了解动物中已鉴定出的数百种miRNA功能的关键一步是确定它们在发育过程中的表达情况。在这里，我们使用锁核酸（LNA）修饰的DNA探针，对斑马鱼胚胎中miRNA原位检测的条件进行了详细分析，并报告了小鼠胚胎中15种miRNA的表达模式。