工厂工人血浆白蛋白的分子量与糖化指数的相关性研究。
Correlation between molecular weight of plasma albumin determined with mass spectrometry and glycation indices in factory workers.
机构信息
Department of Social and Environmental Medicine, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan.
出版信息
Environ Health Prev Med. 2004 Mar;9(2):63-6. doi: 10.1007/BF02897934.
OBJECTIVES
To determine the difference in molecular weights of albumin in factory workers caused by non-enzymatic glycation of plasma albumin using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF/MS), and examine the epidemiological validity of this method.
METHODS
Forty-eight male workers were tested by random sampling. The difference in molecular weights (ΔM) between the subjects' albumin and human serum albumin was determined by MALDI TOF/MS. Correlations between ΔM vs. fructosamine, fasting plasma glucose (FPG) and HbAlc were investigated.
RESULTS
ΔM showed a significant correlation with all of the tested glycation indices (ΔM vs. fructosamine: r=0.487, p<0.001) (ΔM vs. FPG: r=0.482, p<0.01) (ΔM vs. HbAlc: r=0.397, p<0.01).
CONCLUSIONS
Since a significant correlation between ΔM and the glycation indices was found in this investigation, further study with a larger number of subjects is needed for use in clinical applications.
目的
使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF/MS）测定工厂工人血浆白蛋白非酶糖基化引起的白蛋白分子量差异，并检验该方法的流行病学有效性。
方法
采用随机抽样的方法对 48 名男性工人进行检测。MALDI-TOF/MS 测定受试者白蛋白与人血清白蛋白之间的分子量差异（ΔM）。研究 ΔM 与果糖胺、空腹血糖（FPG）和 HbAlc 的相关性。
结果
ΔM 与所有测试的糖化指标均呈显著相关（ΔM 与果糖胺：r=0.487，p<0.001）（ΔM 与 FPG：r=0.482，p<0.01）（ΔM 与 HbAlc：r=0.397，p<0.01）。
结论
本研究发现 ΔM 与糖化指标之间存在显著相关性，因此需要进一步进行更多研究以应用于临床。
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