Lapolla A, Fedele D, Aronica R, Garbeglio M, D'Alpaos M, Seraglia R, Traldi P
Istituto di Medicina Interna, Malattie del Metabolismo, Padova, Italy.
Rapid Commun Mass Spectrom. 1996;10(9):1133-5. doi: 10.1002/(SICI)1097-0231(19960715)10:9<1133::AID-RCM581>3.0.CO;2-C.
Matrix-assisted laser desorption/ionization (MALDI) has been employed for the determination of molecular weights of alpha- and beta-globins obtained from blood samples of healthy and diabetic subjects. Glycated species, originating from the reaction of glucose with the proteins, are easily evident. The MALDI measurements allowed one to distinguish between simple glycation and glyco-oxidation processes of haemoglobin. In fact, together with the products arising from simple glucose condensation on the two proteins, compounds at different oxidation levels were evident. Due to its high specificity, MALDI must be considered a valuable tool for diagnosis in the diabetes field.
基质辅助激光解吸/电离（MALDI）已被用于测定从健康受试者和糖尿病患者血液样本中获得的α-和β-珠蛋白的分子量。由葡萄糖与蛋白质反应产生的糖化产物很容易被发现。MALDI测量能够区分血红蛋白的简单糖化和糖氧化过程。事实上，除了两种蛋白质上简单葡萄糖缩合产生的产物外，不同氧化水平的化合物也很明显。由于其高特异性，MALDI必须被视为糖尿病领域诊断的一种有价值的工具。
