采用微核试验研究慢性暴露于水溶性富勒烯诱导细胞系的遗传毒性。
Genotoxicity in cell lines induced by chronic exposure to water-soluble fullerenes using micronucleus test.
机构信息
Department of Epidemiology, Research Institute, National Cardiovascular Center, 565-8565, Suita, Osaka, Japan,
出版信息
Environ Health Prev Med. 2006 Nov;11(6):292-7. doi: 10.1007/BF02898019.
OBJECTIVES
Nanomaterials have numerous potential benefits for society, but the effects of nanomaterials on human health are poorly understood. In this study, we aim to determine the genotoxic effects of chronic exposure to nanomaterials in various cell lines.
METHODS
Chinese hamster ovary (CHO) cells, human epidermoid-like carcinoma (Hela) cells and human embryonic kidney 293 (HEK293) cells were treated with the water-soluble fullerence C(60)(OH)(24) for 33-80 days. Cell proliferation, cytotoxic analysis and micronucleus tests were performed.
RESULTS
When treated with C(60)(OH)(24) (0, 10, 100, or 1000 pg/ml) for 33 days, both the HEK293 and Hela cells showed increased cell proliferation, but cellular lactate dehydrogenase (LDH) activity was not affected. After long-term exposure (80 days) to C(60)(OH)(24) (0, 10, 100, or 1000 pg/ml), the CHO, Hela and HEK293 cells showed increased genotoxicity on the micronucleus test.
CONCLUSION
This study suggests that nanomaterials, such as C(60)(OH)(24), have genotoxic effects.
目的
纳米材料对社会具有众多潜在益处，但人们对其对人类健康的影响知之甚少。本研究旨在确定纳米材料在各种细胞系中慢性暴露的遗传毒性作用。
方法
用水溶性富勒烯 C（60）（OH）（24）处理中国仓鼠卵巢（CHO）细胞、人表皮样癌细胞（Hela）和人胚肾 293（HEK293）细胞 33-80 天。进行细胞增殖、细胞毒性分析和微核试验。
结果
当用 C（60）（OH）（24）（0、10、100 或 1000 pg/ml）处理 33 天时，HEK293 和 Hela 细胞均显示细胞增殖增加，但细胞乳酸脱氢酶（LDH）活性不受影响。长期暴露（80 天）于 C（60）（OH）（24）（0、10、100 或 1000 pg/ml）后，CHO、Hela 和 HEK293 细胞的微核试验显示遗传毒性增加。
结论
本研究表明，纳米材料（如 C（60）（OH）（24））具有遗传毒性作用。
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