Department of Pathology, Jonsson Comprehensive Cancer Center and Broad Center for Regenerative Medicine and Stem Cell Biology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095-1732, USA.
Prostate. 2011 Nov;71(15):1668-79. doi: 10.1002/pros.21383. Epub 2011 Mar 22.
The majority of the prostatic cancers are adenocarcinomas characterized by glandular formation and the expression of luminal differentiation markers androgen receptor (AR) and prostate-specific antigen (PSA). Most adenocarcinomas are indolent and androgen-dependent. Hormonal therapy that inhibits AR signaling produces symptomatic relief in patients with advanced and metastatic adenocarcinomas. Prostatic small cell neuroendocrine carcinoma (SCNC) is a variant form of prostate cancer (PC). In contrast to adenocarcinoma, the tumor cells of SCNC do not form glands and are negative for AR and PSA. SCNC is extremely aggressive and does not respond to hormonal therapy. The purpose of this study was to compare the important and relevant features of two most commonly used PC cell lines, LNCaP and PC3, with prostatic adenocarcinoma and SCNC.
Xenograft tumors of LNCaP and PC3 were prepared and compared with human prostatic adenocarcinoma and SCNC for the expression of key signaling molecules by immunohistochemistry and Western blot analysis.
LNCaP cells express AR and PSA and their growth is inhibited by androgen withdrawal, similar to human prostatic adenocarcinoma. PC3 cells do not express AR and PSA and their proliferation is independent of androgen, similar to SCNC. Adenocarcinoma cells and LNCaP cells are negative for neuroendocrine markers and stem cell-associated marker CD44 while SCNC and PC3 cells are positive. LNCaP cells have identical cytokeratin profiles to adenocarcinoma while PC3 cells have cytokeratin profiles similar to SCNC.
LNCaP cells share common features with adenocarcinoma while PC3 cells are characteristic of SCNC.
大多数前列腺癌是腺癌，其特征是腺体形成和管腔分化标志物雄激素受体 (AR) 和前列腺特异性抗原 (PSA) 的表达。大多数腺癌是惰性的和雄激素依赖性的。抑制 AR 信号的激素治疗可缓解晚期和转移性腺癌患者的症状。前列腺小细胞神经内分泌癌 (SCNC) 是前列腺癌 (PC) 的一种变体。与腺癌不同，SCNC 的肿瘤细胞不形成腺体，并且 AR 和 PSA 均为阴性。SCNC 具有极强的侵袭性，且对激素治疗无反应。本研究的目的是比较两种最常用的 PC 细胞系 LNCaP 和 PC3 与前列腺腺癌和 SCNC 的重要和相关特征。
制备 LNCaP 和 PC3 的异种移植肿瘤，并通过免疫组织化学和 Western blot 分析与人类前列腺腺癌和 SCNC 进行比较，以检测关键信号分子的表达。
LNCaP 细胞表达 AR 和 PSA，其生长受雄激素剥夺抑制，类似于人类前列腺腺癌。PC3 细胞不表达 AR 和 PSA，其增殖不受雄激素影响，类似于 SCNC。腺癌细胞和 LNCaP 细胞均为神经内分泌标志物和干细胞相关标志物 CD44 阴性，而 SCNC 和 PC3 细胞均为阳性。LNCaP 细胞的细胞角蛋白谱与腺癌相同，而 PC3 细胞的细胞角蛋白谱与 SCNC 相似。
LNCaP 细胞与腺癌具有共同特征，而 PC3 细胞是 SCNC 的特征。
