用于构建梳状肽-聚合物生物共轭物的三唑形成生物共轭技术比较。
A comparison of triazole-forming bioconjugation techniques for constructing comb-shaped peptide-polymer bioconjugates.
作者信息
Canalle Luiz A, van der Knaap Matthijs, Overhand Mark, van Hest Jan C M
机构信息
Institute for Molecules and Materials, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.
出版信息
Macromol Rapid Commun. 2011 Jan 17;32(2):203-8. doi: 10.1002/marc.201000507. Epub 2010 Nov 15.
The grafting-to of a peptide to a side chain functional polymer was investigated using "click" chemistry. Two click reactions were compared: the copper-free strain-promoted azide-alkyne 1,3-cycloaddition (SPAAC) and the traditional copper-catalyzed azide-alkyne 1,3-cycloaddition (CuAAC). For the resulting comb-shaped products, it was found that the steric bulk of the conjugation moiety used in SPAAC limits the degree of grafting for these highly dense systems, whereas CuAAC gives (near) quantitative functionalization.
利用“点击”化学研究了肽与侧链功能聚合物的接枝。比较了两种点击反应：无铜应变促进的叠氮化物-炔烃1,3-环加成反应（SPAAC）和传统的铜催化叠氮化物-炔烃1,3-环加成反应（CuAAC）。对于所得的梳状产物，发现SPAAC中使用的共轭部分的空间位阻限制了这些高密度体系的接枝程度，而CuAAC则能实现（近乎）定量功能化。
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