Vutto N L, Gapeeva T A, Pundik A N, Tret'iakova T G, Volotovskiĭ I D
Genetika. 2010 Dec;46(12):1626-34.
Binary vectors for Agrobacterium-mediated transformation were constructed to express the genes for antimicrobial peptides (APs) of the cectropin-melittin type under the control of the cauliflower mosaic virus 35S RNA promoter in plants. It was shown with Escherichia coli and Agrobacterium tumefaciens cells that the cassettes could be cloned in pB1121-based vectors with deletion of the 3-D-glycuronidase gene only in the orientation opposite to that of the original vector. Transgenic potato plants were obtained using the Belarussian varieties Odyssey, Vetraz, and Scarb. Their cells expressed the MsrA1 or CEMA peptides of the cecropin-melittin type. The expression was shown to confer higher resistance to bacterial (Erwinia carotovora) infection and extremely high resistance to fungal (Phytophtora infestans and Alternarla solani) infections.
构建了用于农杆菌介导转化的二元载体，以在植物中花椰菜花叶病毒35S RNA启动子的控制下表达天蚕素-蜂毒素型抗菌肽（AP）的基因。用大肠杆菌和根癌农杆菌细胞证明，这些盒式结构可以克隆到基于pB1121的载体中，仅在与原始载体相反的方向上缺失β-葡萄糖醛酸酶基因。使用白俄罗斯品种奥德赛、韦特拉兹和斯卡布获得了转基因马铃薯植株。它们的细胞表达了天蚕素-蜂毒素型的MsrA1或CEMA肽。结果表明，这种表达赋予了对细菌（胡萝卜软腐欧文氏菌）感染的更高抗性以及对真菌（致病疫霉和茄链格孢）感染的极高抗性。
