Wen Hai-Tao, Zhao Hong-Ying, Lin Li, Fang Gui-Fang
College of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Zhong Yao Cai. 2010 Nov;33(11):1686-9.
Cloned several important functional genes from Citrus grandis 'tomentosa', which was the genuine medicinal materials from Guangdong province.
CTAB-LiCl treatment was used to extract RNA from Citrus grandis 'tomentosa', the technology of RT-PCR was used to clone the genes.
Three genes were cloned from Citrus grandis 'tomentosa', which were Chalcone Synthase (CHS) gene, Flavanone 3-hydroxylase (F3H) gene and Chalcone isomerase (CHI) gene. The open reading frame (ORF) of CHS gene was consisted of 1173 bp, and encoded 391 amino acid residues, that of F3H gene was consisted of 1086 bp, and encoded 362 amino acid residues, that of CHI gene was consisted of 666 bp, and encoded 222 amino acid residues. The accession number of three genes were GQ892059, GU323284 and GU323285 respectively.
CTAB-LiCl treatment could extract high quality RNA from Citrus grandis 'tomentosa', which could be used to follow-up work. The three functional genes which were cloned from Citrus grandis 'tomentosa' had ORFs.
从广东省地道药材化州柚中克隆几个重要的功能基因。
采用CTAB-LiCl法从化州柚中提取RNA，运用RT-PCR技术克隆基因。
从化州柚中克隆到3个基因，分别为查尔酮合酶（CHS）基因、黄烷酮3-羟化酶（F3H）基因和查尔酮异构酶（CHI）基因。CHS基因的开放阅读框（ORF）由1173 bp组成，编码391个氨基酸残基；F3H基因的ORF由1086 bp组成，编码362个氨基酸残基；CHI基因的ORF由666 bp组成，编码222个氨基酸残基。这3个基因的登录号分别为GQ892059、GU323284和GU323285。
CTAB-LiCl法能从化州柚中提取高质量的RNA，可用于后续研究。从化州柚中克隆到的3个功能基因具有开放阅读框。
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