PCR 扩增和克隆参与橙皮苷生物合成的酪氨酸脱羧酶。

PCR amplification and cloning of tyrosine decarboxylase involved in synephrine biosynthesis in Citrus.

机构信息

Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 800 Buchanan St., Albany, CA 94710, USA.

出版信息

N Biotechnol. 2010 Sep 30;27(4):308-16. doi: 10.1016/j.nbt.2010.04.003. Epub 2010 Apr 18.

DOI:10.1016/j.nbt.2010.04.003

PMID:20403465

Abstract

BACKGROUND

The phenolic amine synephrine is a vascoconstrictor and bronchiectatic agent and holds promise as an aid to weight management and obesity reduction. Synephrine is structurally similar to the active ingredients of several commercial cold remedies. Some Citrus contain high concentrations of synephrine. An enzyme involved in synephrine biosynthesis, tyrosine decarboxylase (TYDC), is a pyridoxal-5'-phosphate (PLP)-dependent enzyme that decarboxylates tyrosine to yield CO(2) and tyramine. We used PCR to screen, clone and sequence this gene from various synephrine producing and nonproducing Citrus species and varieties to determine if DNA sequence of this gene correlated with synephrine presence.

RESULTS

PCR amplification and comparison of DNA sequence indicates DNA sequence differences that may cause production of truncated proteins to be produced in some nonsynephrine producing Citrus.

CONCLUSION

Synephrine production may be genetically determined in part by the gene for TYDC.

摘要

背景

酚胺辛弗林是一种血管收缩剂和支气管扩张剂，有望成为辅助体重管理和减肥的手段。辛弗林在结构上与几种商业感冒药的有效成分相似。一些柑橘类水果含有高浓度的辛弗林。参与辛弗林生物合成的酶，酪氨酸脱羧酶（TYDC），是一种依赖于吡哆醛-5'-磷酸（PLP）的酶，它使酪氨酸脱羧生成 CO（2）和酪胺。我们使用 PCR 从各种产生和不产生辛弗林的柑橘属物种和品种中筛选、克隆和测序该基因，以确定该基因的 DNA 序列是否与辛弗林的存在相关。