Sheng Shu-Jing, Yan Ping, Zheng Chuan-Jin, Zhao Shu-Jin
Department of Pharmacy, General Hospital of Guangzhou Military Command, Guangzhou 510010, China.
Zhong Yao Cai. 2010 Nov;33(11):1707-11.
To identify Fallopia multiflora from its adulterants by comparing their matK sequences.
Genomic DNA of different materials was extracted using modified cetytrimethyl ammonium bromide (CTAB) method. The double-strand matK genes were amplified using PCR method and then sequenced. The data were analyzed in Clustral W and MEGA 4.0 software package.
Besides F. multiflora var. ciliinerve, the matK sequences of other adulterants show distinct differences with F. multiflora, whether for nucleotides substitutions or genetic distances; and the specific identifying sites for distinguishing F. multiflora and other Fallopia adulterants were found through further comparative analysis. Moreover, the 3 inspected materials were successfully authenticated by comparing the matK sequences.
matK sequences can be used for the molecular identification between F. multiflora and its adulterant species.
通过比较何首乌与其伪品的matK序列来鉴别何首乌。
采用改良十六烷基三甲基溴化铵（CTAB）法提取不同材料的基因组DNA。用PCR法扩增双链matK基因，然后进行测序。数据在Clustral W和MEGA 4.0软件包中进行分析。
除毛脉蓼外，其他伪品的matK序列与何首乌相比，无论是核苷酸替换还是遗传距离都存在明显差异；通过进一步比较分析，找到了区分何首乌与其他虎杖属伪品的特异性鉴别位点。此外，通过比较matK序列成功鉴定了3种被检材料。
matK序列可用于何首乌与其伪品之间的分子鉴定。
