通过彗星试验评估墨西哥宫颈发育异常女性的DNA损伤。
DNA damage in Mexican women with cervical dysplasia evaluated by comet assay.
作者信息
Cortés-Gutiérrez Elva I, Dávila-Rodríguez Martha I, Zamudio-González Estela A, Aguado-Barrera Miguel E, Vargas-Villarreal Javier, Cerda-Flores Ricardo M
机构信息
Division of Genetics, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Universidad Autónoma de Nuevo León, Monterrey, Mexico.
出版信息
Anal Quant Cytol Histol. 2010 Aug;32(4):207-13.
OBJECTIVE
To investigate the association between the progressive stage of cervical dysplasia and DNA damage by comet assay.
STUDY DESIGN
A hospital-based, unmatched, case-control study was performed. DNA damage levels (none, low, medium and high) in the cervical epithelial cells of 31 women (10 with low grade squamous intraepithelial lesions [LSIL], 10 with high grade [HSIL] and 11 with no cervical lesion) were evaluated using the comet assay.
RESULTS
A significant increase in medium DNA damage was observed in women with HSIL (17 +/- 8.9) relative to that in the control women (9 +/- 6.1). A significant increase in high DNA damage was also observed in women with LSIL (23 +/- 15.4) or HSIL (32 +/- 13.1) relative to that in the control women (12 +/- 7.9).
CONCLUSION
These findings confirm that the grade of a cervical lesion correlates with the degree of genomic instability.
目的
通过彗星试验研究宫颈发育异常进展阶段与DNA损伤之间的关联。
研究设计
进行了一项基于医院的非匹配病例对照研究。使用彗星试验评估了31名女性（10名低级别鳞状上皮内病变[LSIL]、10名高级别[HSIL]和11名无宫颈病变）宫颈上皮细胞中的DNA损伤水平（无、低、中、高）。
结果
相对于对照女性（9±6.1），HSIL女性（17±8.9）中观察到中度DNA损伤显著增加。相对于对照女性（12±7.9），LSIL女性（23±15.4）或HSIL女性（32±13.1）中也观察到高度DNA损伤显著增加。
结论
这些发现证实宫颈病变的分级与基因组不稳定性程度相关。
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