异常的p16甲基化是宫颈鳞状细胞癌发生过程中烟草暴露的生物标志物。

Aberrant p16 methylation is a biomarker for tobacco exposure in cervical squamous cell carcinogenesis.

作者信息

Lea Jayanthi S, Coleman Robert, Kurien Aleena, Schorge John O, Miller David S, Minna John D, Muller Carolyn Y

机构信息

Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, and the Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas, Texas, USA.

出版信息

Am J Obstet Gynecol. 2004 Mar;190(3):674-9. doi: 10.1016/j.ajog.2003.09.036.

DOI:10.1016/j.ajog.2003.09.036

PMID:15041998

Abstract

OBJECTIVE

The purpose of this study was to determine the association between active tobacco exposure and aberrant p16 promoter methylation in primary cervical squamous cell cancer and high-grade squamous cervical dysplasia.

STUDY DESIGN

p16 methylation-specific polymerase chain reaction was performed on DNA that was extracted from 60 cervical cancers, 30 high-grade dysplasia specimens, and 78 normal cervical cytologic specimens. Patient data were obtained by medical record review or were collected prospectively.

RESULTS

Aberrant p16 methylation was significantly higher in squamous cell cervical cancers (61%) than in squamous high-grade dysplasia (20%) or normal cytologic specimens (7.5%). Approximately one half the women with squamous cancer and one half of the women with high-grade dysplasia were active smokers. Aberrant p16 methylation was associated with active tobacco use in patients with squamous carcinoma (odds ratio, 20.6; 95% CI, 3.6-118; P<.001) and high-grade dysplasia (odds ratio, 4.57; 95% CI, 1.63-12.78; P=.002).

CONCLUSION

Aberrant p16 methylation is associated strongly with active tobacco use in squamous cell cervical cancers and high-grade dysplasia.

摘要

目的

本研究旨在确定原发性宫颈鳞状细胞癌和高级别宫颈鳞状上皮发育异常中主动吸烟暴露与p16启动子异常甲基化之间的关联。

研究设计

对从60例宫颈癌、30例高级别发育异常标本和78例正常宫颈细胞学标本中提取的DNA进行p16甲基化特异性聚合酶链反应。通过病历审查获取患者数据或前瞻性收集。

结果

宫颈鳞状细胞癌中异常p16甲基化（61%）显著高于鳞状高级别发育异常（20%）或正常细胞学标本（7.5%）。约一半的鳞状细胞癌女性患者和一半的高级别发育异常女性患者为主动吸烟者。鳞状细胞癌患者（优势比，20.6；95%可信区间，3.6 - 118；P <.001）和高级别发育异常患者（优势比，4.57；95%可信区间，1.63 - 12.78；P =.002）中，异常p16甲基化与主动吸烟有关。