Chifiriuc Mariana Carmen, Bleotu Coralia, Pîrcălăbioru Gratiela, Israil Anca Michaela, Dinu Sorin, Rută Simona Maria, Grancea Camelia, Lazăr Veronica
University of Bucharest, Faculty of Biology, Microbiology Immunology Department, Aleea Portocalelor, 1-5,Bucharest, Romania.
Roum Arch Microbiol Immunol. 2010 Jul-Sep;69(3):164-72.
Vibrio (V.) parahaemolyticus is an aquatic halophilic bacteria which produces gastroenteritis and in rare cases septicaemia after the consumption of raw or under-cooked contaminated seafood.The severity of diarrheal illness caused by this bacterium is closely related to the presence of two types of hemolysins (the thermostable direct hemolysin-TDH and TDH related hemolysin-TRH) and also of type III secretion system (TTSS) proteins. The TTSS type 1 induces a wide array of effects on infected HeLa cells such as autophagy, oncosis, cell rounding and lysis. Previous studies have shown that heat shock proteins have the ability to stimulate the production of interleukins in different cellular cultures. In our studies we have stimulated two cellular lines (HeLa and human diploid cells) with different V. parahaemolyticus culture fractions in order to observe the effect on cytokines production. Thus, the purpose of this study was to analyze the expression of IL-1, IL-2, IL-4, IL-6, IL-10 and TNF-alpha induced by the cell treatment with total cellular lysate, periplasmic fractions and culture supernatants extracted from V. parahaemolyticus exposed to normal and also to stress conditions. The ELISA assay of the cytokine profile of the HeLa and HDC cell lines stimulated with different bacterial fractions revealed that in the V. parahemolyticus cultures submitted to osmotic and heat shock stress are accumulating factors (probably heat shock proteins) which are exhibiting immunomodulatory activity, responsible for the induction of a pro-inflammatory response associated with increased levels of IL-6 and TNF-alpha expression, however balanced by the stimulation of the anti-inflammatory cytokine IL-4 synthesis.
副溶血性弧菌是一种嗜盐性水生细菌，食用受污染的生的或未煮熟的海鲜后会引发肠胃炎，在极少数情况下会导致败血症。这种细菌引起的腹泻疾病的严重程度与两种溶血素（耐热直接溶血素 - TDH和TDH相关溶血素 - TRH）以及III型分泌系统（TTSS）蛋白的存在密切相关。1型TTSS对受感染的HeLa细胞会产生一系列影响，如自噬、胀亡、细胞变圆和裂解。先前的研究表明，热休克蛋白有能力在不同的细胞培养物中刺激白细胞介素的产生。在我们的研究中，我们用不同的副溶血性弧菌培养组分刺激了两种细胞系（HeLa细胞和人二倍体细胞），以观察对细胞因子产生的影响。因此，本研究的目的是分析用从处于正常和应激条件下的副溶血性弧菌中提取的全细胞裂解物、周质组分和培养上清液处理细胞后诱导的IL-1、IL-2、IL-4、IL-6、IL-10和TNF-α的表达。用不同细菌组分刺激HeLa和HDC细胞系的细胞因子谱的ELISA分析表明，在经历渗透和热休克应激的副溶血性弧菌培养物中积累了一些因子（可能是热休克蛋白），这些因子具有免疫调节活性，负责诱导与IL-6和TNF-α表达水平升高相关的促炎反应，不过这种反应因抗炎细胞因子IL-4合成的刺激而得到平衡。
