不同 VGLUT 异构体与内收蛋白 A1 的相互作用调节神经递质释放和短期可塑性。
Interplay between VGLUT isoforms and endophilin A1 regulates neurotransmitter release and short-term plasticity.
机构信息
Departments of Neuroscience and Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
出版信息
Neuron. 2011 Mar 24;69(6):1147-59. doi: 10.1016/j.neuron.2011.02.002.
Vesicular glutamate transporters (VGLUTs) are essential for filling synaptic vesicles with glutamate and mammals express three VGLUT isoforms (VGLUT1-3) with distinct spatiotemporal expression patterns. Here, we find that neurons expressing VGLUT1 have lower release probability and less short-term depression than neurons expressing VGLUT2 or VGLUT3. Investigation of the underlying mechanism identified endophilin A1 as a positive regulator of exocytosis whose expression levels are positively correlated with release efficiency and showed that the differences in release efficiency between VGLUT1- and VGLUT2-expressing neurons are due to VGLUT1's ability to bind endophilin A1 and inhibit endophilin-induced enhancement of release probability.
囊泡谷氨酸转运体（VGLUTs）对于将谷氨酸填充到突触小泡中至关重要，哺乳动物表达三种 VGLUT 同工型（VGLUT1-3），具有不同的时空表达模式。在这里，我们发现表达 VGLUT1 的神经元的释放概率低于表达 VGLUT2 或 VGLUT3 的神经元，并且其短期抑制程度也较低。对潜在机制的研究确定了内收蛋白 A1 是胞吐作用的正调节剂，其表达水平与释放效率呈正相关，并表明表达 VGLUT1 和 VGLUT2 的神经元之间的释放效率差异是由于 VGLUT1 能够与内收蛋白 A1 结合并抑制内收蛋白诱导的释放概率增强。
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