视网膜母细胞瘤中高危型人乳头瘤病毒基因型的流行情况。

Prevalence of high-risk human papillomavirus genotypes in retinoblastoma.

机构信息

Department of Pathology, Kidwai Memorial Institute of Oncology, Dr. M.H. Marigowda Road, Bangalore 560 029, Karnataka, India.

出版信息

Br J Ophthalmol. 2011 Jul;95(7):1014-8. doi: 10.1136/bjo.2010.199802. Epub 2011 Mar 24.

DOI:10.1136/bjo.2010.199802

PMID:21436174

Abstract

BACKGROUND

The human papillomavirus (HPV) is an important aetiological agent in cancer but its involvement in retinoblastomas (RBs) is controversial.

METHODS

64 formalin-fixed paraffin-embedded tissue blocks and 19 fresh-frozen specimens were subjected to multiplex PCR using PGMY09/11 primers, HPV genotyping, non-isotopic in situ hybridisation and immunohistochemistry for pRb and p16(INK4a).

RESULTS

24% of RBs contained HPV DNA. 90% of HPV genotypes were of high-risk (HR) type and 10% were of intermediate-risk (IR) type. HR HPVs 45, 59, 68 and 52 were detected for the first time, as were IR HPVs 82 and 73. There was only one HPV 18-positive case. Interestingly, no low-risk genotypes were identified. Nine formalin-fixed paraffin-embedded HPV-positive cases showed nuclear HPV positivity by non-isotopic in situ hybridisation. Immunohistochemistry did not show pRb expression in 67% of cases. 34% expressed nuclear p16(INK4a), of which 20 cases were also positive for HPV by multiplex PCR. A statistically significant association between HPV and pRb expression status was observed (p=0.0001).The association of HPV with p16(INK4a) expression was also statistically significant (p=0.0001).

CONCLUSIONS

While the presence of HPV in a subset of RB was demonstrated, its role in carcinogenesis needs further elucidation.

摘要

背景

人乳头瘤病毒（HPV）是癌症的重要病因，但它在视网膜母细胞瘤（RB）中的作用仍存在争议。

方法

使用 PGMY09/11 引物对 64 个福尔马林固定石蜡包埋组织块和 19 个新鲜冷冻标本进行多重 PCR，HPV 基因分型，非同位素原位杂交和 pRb 和 p16(INK4a)的免疫组织化学。

结果

24%的 RB 含有 HPV DNA。90%的 HPV 基因型为高危型（HR），10%为中危型（IR）。首次检测到 HR HPV 45、59、68 和 52，以及 IR HPV 82 和 73。只有一个 HPV 18 阳性病例。有趣的是，没有发现低危型。9 例福尔马林固定石蜡包埋 HPV 阳性病例通过非同位素原位杂交显示核 HPV 阳性。免疫组织化学显示 67%的病例中 pRb 表达缺失。34%表达核 p16(INK4a)，其中 20 例通过多重 PCR 也呈 HPV 阳性。HPV 与 pRb 表达状态之间存在统计学显著相关性（p=0.0001）。HPV 与 p16(INK4a)表达之间也存在统计学显著相关性（p=0.0001）。