[液相色谱-质谱法测定黄连和黄柏中的木兰碱]

[Determination of magnoflorine in Coptidis Rhizoma and Phellodendri Chinensis Cortex by LC-MS].

作者信息

Fan Shengjun, Yu Dahai, Gu Yuanqinl, Zhang Mingyu, Zhang Lin, Li Guoyu

机构信息

Department of Pharmacology, Harbin Medical University, Harbin 150086, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2010 Dec;35(24):3322-4.

PMID:21438400

Abstract

A rapid and specific high performance liquid chromatography-mass spectrometric method was developed for determination of magnoflorine in Rhizoma Coptidis and Cortex Phellodendri Chinensis. Samples were extracted by methanol. Agilent Eclipse XDB-C18 ODS column (4.6 mm x 150 mm, 5 microm) was used, and mobile phase was methanol-water (60:40) at a flow rate of 0.8 mL x min(-1). Electrospray ionization model (ESI), and MRM model were used for quantification. The linear range of magnoflorine was 4.352-2720 microg x L(-1). The average recovery was above 98%. The method is simple, sensitive and accurate, it can be used for determination of magnoflorine in Rhizoma Coptidis and Cortex Phellodendri Chinensis.

摘要

建立了一种快速、特异的高效液相色谱-质谱法用于测定黄连和黄柏中的小檗碱。样品用甲醇提取。采用安捷伦Eclipse XDB-C18 ODS柱（4.6 mm×150 mm，5μm），流动相为甲醇-水（60:40），流速为0.8 mL·min⁻¹。采用电喷雾电离模式（ESI）和多反应监测模式（MRM）进行定量分析。小檗碱的线性范围为4.352 - 2720μg·L⁻¹。平均回收率在98%以上。该方法简便、灵敏、准确，可用于测定黄连和黄柏中的小檗碱。