实验性中风后小鼠大脑中 T 淋巴细胞的体内实时多光子成像。
In vivo real-time multiphoton imaging of T lymphocytes in the mouse brain after experimental stroke.
机构信息
Strathclyde Institute of Pharmacy & Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow G4 0RE, UK.
出版信息
Stroke. 2011 May;42(5):1429-36. doi: 10.1161/STROKEAHA.110.603704. Epub 2011 Mar 24.
BACKGROUND AND PURPOSE
To gain a better understanding of T cell behavior after stroke, we have developed real-time in vivo brain imaging of T cells by multiphoton microscopy after middle cerebral artery occlusion.
METHODS
Adult male hCD2-GFP transgenic mice that exhibit green fluorescent protein-labeled T cells underwent permanent left distal middle cerebral artery occlusion by electrocoagulation (n=6) or sham surgery (n=6) and then multiphoton laser imaging 72 hours later.
RESULTS
Extravasated T cell number significantly increased after middle cerebral artery occlusion versus sham. Two T cell populations existed after middle cerebral artery occlusion, possibly driven by 2 T cell subpopulations; 1 had significantly lower and the other significantly higher track velocity and displacement rate than sham.
CONCLUSIONS
The different motilities and behaviors of T cells observed using our imaging approach after stroke could reveal important mechanisms of immune surveillance for future therapeutic exploitations.
背景与目的
为了更好地了解中风后 T 细胞的行为，我们通过多光子显微镜开发了一种实时在体脑内 T 细胞成像方法，用于大脑中动脉闭塞后的研究。
方法
成年雄性 hCD2-GFP 转基因小鼠（其 T 细胞表达绿色荧光蛋白）通过电凝法进行永久性左侧大脑中动脉闭塞（n=6）或假手术（n=6），然后在 72 小时后进行多光子激光成像。
结果
与假手术组相比，大脑中动脉闭塞后，细胞外渗 T 细胞数量明显增加。大脑中动脉闭塞后存在两种 T 细胞群，可能由两种 T 细胞亚群驱动；1 种 T 细胞的轨迹速度和位移率明显低于假手术组，而另 1 种则明显高于假手术组。
结论
我们的成像方法观察到中风后 T 细胞的不同运动性和行为特征，可能揭示了免疫监视的重要机制，为未来的治疗开发提供了依据。
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