Ioannou Ioannis, Sandalakis Vassilios, Kassinis Nikos, Chochlakis Dimosthenis, Papadopoulos Byron, Loukaides Fedias, Tselentis Yannis, Psaroulaki Anna
Veterinary Services, 1417, Nicosia, Cyprus.
J Wildl Dis. 2011 Apr;47(2):300-6. doi: 10.7589/0090-3558-47.2.300.
The Cypriot mouflon (Ovis orientalis ophion), a once almost extirpated species of wild sheep, is under strict surveillance because it can be threatened by likely transmission of pathogenic bacteria, such as Anaplasma spp., Rickettsia spp., and Coxiella burnetii, primarily from domestic ungulates. We collected 77 blood samples from Cypriot mouflons and 663 of their ectoparasites (Rhipicephalus turanicus, Rhipicephalus sanguineus, Rhipicephalus bursa, Hyalomma anatolicum excavatum, Hyalomma marginatum, Haemaphysalis punctata, Haemaphysalis sulcata, and Ixodes gibossus) and tested them by polymerase chain reaction and sequencing. Twenty-three mouflon blood samples (30%) were positive for C. burnetii, 23 (30%) for Rickettsia spp., and 8 (10%) for Anaplasma ovis. Of 109 pools of ectoparasites, 32.1% were positive for C. burnetii, 28.4% for Rickettsia spp., and 10.9% for A. ovis; 11.9% were positive for both C. burnetii and Rickettsia spp., 6.4% for both Rickettsia spp. and A. ovis, and 2.8% for all three pathogens. This is the first survey that records the presence of tick-borne pathogens, both in the Cypriot mouflon and in ticks parasitizing it.
塞浦路斯摩弗伦羊（东方盘羊塞浦路斯亚种，Ovis orientalis ophion）是一种曾几近灭绝的野生绵羊，由于可能受到主要来自家养有蹄类动物的致病细菌（如无形体属、立克次氏体属和伯氏考克斯氏体）传播的威胁，正处于严格监测之下。我们从塞浦路斯摩弗伦羊身上采集了77份血液样本及其663只体外寄生虫（图兰扇头蜱、血红扇头蜱、囊形扇头蜱、边缘璃眼蜱、小亚璃眼蜱、点状血蜱、苏氏血蜱和驼背硬蜱），并通过聚合酶链反应和测序进行检测。23份摩弗伦羊血液样本（30%）检测出伯氏考克斯氏体呈阳性，23份（30%）检测出立克次氏体属呈阳性，8份（10%）检测出绵羊无形体呈阳性。在109组体外寄生虫样本中，32.1%检测出伯氏考克斯氏体呈阳性，28.4%检测出立克次氏体属呈阳性，10.9%检测出绵羊无形体呈阳性；11.9%同时检测出伯氏考克斯氏体和立克次氏体属呈阳性，6.4%同时检测出立克次氏体属和绵羊无形体呈阳性，2.8%三种病原体检测均呈阳性。这是首次记录塞浦路斯摩弗伦羊及其寄生蜱中存在蜱传病原体的调查。
