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本文引用的文献

1
Quantification of Shiga toxin-converting bacteriophages in wastewater and in fecal samples by real-time quantitative PCR.应用实时定量 PCR 技术对污水和粪便样本中志贺毒素转化噬菌体进行定量分析。
Appl Environ Microbiol. 2010 Sep;76(17):5693-701. doi: 10.1128/AEM.00107-10. Epub 2010 Jul 9.
2
Development and validation of a qPCR-based method for quantifying Shiga toxin-encoding and other lambdoid bacteriophages.基于 qPCR 的方法用于定量检测产志贺毒素编码噬菌体和其他λ噬菌体的开发与验证。
Environ Microbiol. 2010 May;12(5):1194-204. doi: 10.1111/j.1462-2920.2010.02162.x. Epub 2010 Feb 9.
3
High-throughput method for rapid induction of prophages from lysogens and its application in the study of Shiga Toxin-encoding Escherichia coli strains.高通量方法快速诱导溶源菌噬菌体及其在志贺毒素编码大肠杆菌菌株研究中的应用。
Appl Environ Microbiol. 2010 Apr;76(7):2360-5. doi: 10.1128/AEM.02923-09. Epub 2010 Feb 5.
4
Quantification of Shiga toxin 2-encoding bacteriophages, by real-time PCR and correlation with phage infectivity.实时 PCR 定量检测志贺毒素 2 编码噬菌体及其与噬菌体感染力的相关性。
J Appl Microbiol. 2010 Mar;108(3):1105-1114. doi: 10.1111/j.1365-2672.2010.04664.x. Epub 2010 Jan 11.
5
Pathogenic potential and horizontal gene transfer in ovine gastrointestinal Escherichia coli.绵羊胃肠道大肠杆菌的致病潜力和水平基因转移。
J Appl Microbiol. 2010 May;108(5):1552-62. doi: 10.1111/j.1365-2672.2009.04575.x. Epub 2009 Oct 7.
6
Phage-mediated Shiga toxin 2 gene transfer in food and water.噬菌体介导的志贺毒素2基因在食物和水中的转移
Appl Environ Microbiol. 2009 Mar;75(6):1764-8. doi: 10.1128/AEM.02273-08. Epub 2009 Jan 23.
7
Quantitative real-time PCR is not more sensitive than "conventional" PCR.定量实时聚合酶链反应并不比“传统”聚合酶链反应更灵敏。
J Clin Microbiol. 2008 Jun;46(6):1897-900. doi: 10.1128/JCM.02258-07. Epub 2008 Apr 9.
8
Differential expression of stx2 variants in Shiga toxin-producing Escherichia coli belonging to seropathotypes A and C.志贺毒素产生大肠杆菌中stx2变体在血清致病型A和C中的差异表达。
Microbiology (Reading). 2008 Jan;154(Pt 1):176-186. doi: 10.1099/mic.0.2007/009704-0.
9
Multilocus characterization scheme for shiga toxin-encoding bacteriophages.产志贺毒素噬菌体的多位点特征分析方案
Appl Environ Microbiol. 2007 Dec;73(24):8032-40. doi: 10.1128/AEM.01278-07. Epub 2007 Oct 19.
10
Insertion site occupancy by stx2 bacteriophages depends on the locus availability of the host strain chromosome.stx2噬菌体的插入位点占有率取决于宿主菌株染色体的基因座可用性。
J Bacteriol. 2007 Sep;189(18):6645-54. doi: 10.1128/JB.00466-07. Epub 2007 Jul 20.

定量评估牛肉和沙拉中携带志贺毒素的噬菌体的感染性。

Quantification and evaluation of infectivity of shiga toxin-encoding bacteriophages in beef and salad.

机构信息

Department of Microbiology, University of Barcelona, Diagonal 645, Annex, Floor 0, 08028 Barcelona, Spain.

出版信息

Appl Environ Microbiol. 2011 May;77(10):3536-40. doi: 10.1128/AEM.02703-10. Epub 2011 Mar 25.

DOI:10.1128/AEM.02703-10
PMID:21441341
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3126450/
Abstract

Stx bacteriophages in 68 samples of beef and salad were quantified by real-time quantitative PCR (qPCR). Stx phages from the samples were propagated in Escherichia coli C600, E. coli O157:H7, and Shigella strains and further quantified. Fifty percent of the samples carried infectious Stx phages that were isolated from plaques generated by lysis.

摘要

对 68 份牛肉和沙拉样本中的 Stx 噬菌体进行了实时定量 PCR（qPCR）定量。从样本中提取的 Stx 噬菌体在大肠杆菌 C600、大肠杆菌 O157:H7 和志贺氏菌菌株中繁殖，并进一步定量。50%的样本携带可分离的感染性 Stx 噬菌体，这些噬菌体是由裂解形成的噬菌斑中分离得到的。