定量评估牛肉和沙拉中携带志贺毒素的噬菌体的感染性。
Quantification and evaluation of infectivity of shiga toxin-encoding bacteriophages in beef and salad.
机构信息
Department of Microbiology, University of Barcelona, Diagonal 645, Annex, Floor 0, 08028 Barcelona, Spain.
出版信息
Appl Environ Microbiol. 2011 May;77(10):3536-40. doi: 10.1128/AEM.02703-10. Epub 2011 Mar 25.
Abstract
Stx bacteriophages in 68 samples of beef and salad were quantified by real-time quantitative PCR (qPCR). Stx phages from the samples were propagated in Escherichia coli C600, E. coli O157:H7, and Shigella strains and further quantified. Fifty percent of the samples carried infectious Stx phages that were isolated from plaques generated by lysis.
摘要
对 68 份牛肉和沙拉样本中的 Stx 噬菌体进行了实时定量 PCR（qPCR）定量。从样本中提取的 Stx 噬菌体在大肠杆菌 C600、大肠杆菌 O157:H7 和志贺氏菌菌株中繁殖，并进一步定量。50%的样本携带可分离的感染性 Stx 噬菌体，这些噬菌体是由裂解形成的噬菌斑中分离得到的。
相似文献
1
Quantification and evaluation of infectivity of shiga toxin-encoding bacteriophages in beef and salad.定量评估牛肉和沙拉中携带志贺毒素的噬菌体的感染性。
Appl Environ Microbiol. 2011 May;77(10):3536-40. doi: 10.1128/AEM.02703-10. Epub 2011 Mar 25.
2
Isolation and Characterization of Shiga Toxin Bacteriophages.志贺毒素噬菌体的分离与鉴定。
Methods Mol Biol. 2021;2291:119-144. doi: 10.1007/978-1-0716-1339-9_5.
3
Toxigenic properties and stx phage characterization of Escherichia coli O157 isolated from animal sources in a developing country setting.从发展中国家动物源分离的产毒特性及志贺样毒素噬菌体特性的大肠杆菌 O157 研究
BMC Microbiol. 2018 Aug 31;18(1):98. doi: 10.1186/s12866-018-1235-3.
4
Shiga toxin-encoding bacteriophages--genomes in motion.志贺毒素编码噬菌体——动态基因组
Int J Med Microbiol. 2004 Sep;294(2-3):115-21. doi: 10.1016/j.ijmm.2004.06.023.
5
Escherichia coli O157:H7 Shiga toxin-encoding bacteriophages: integrations, excisions, truncations, and evolutionary implications.大肠杆菌O157:H7志贺毒素编码噬菌体：整合、切除、截短及进化意义
J Bacteriol. 2003 Jun;185(12):3596-605. doi: 10.1128/JB.185.12.3596-3605.2003.
6
Shiga toxin 2-encoding bacteriophages in human fecal samples from healthy individuals.健康个体粪便样本中的志贺毒素 2 编码噬菌体。
Appl Environ Microbiol. 2013 Aug;79(16):4862-8. doi: 10.1128/AEM.01158-13. Epub 2013 Jun 7.
7
Diversity and host range of Shiga toxin-encoding phage.产志贺毒素噬菌体的多样性及宿主范围
Infect Immun. 2004 Dec;72(12):7131-9. doi: 10.1128/IAI.72.12.7131-7139.2004.
8
Diversity of stx2 converting bacteriophages induced from Shiga-toxin-producing Escherichia coli strains isolated from cattle.从牛源产志贺毒素大肠杆菌菌株中诱导出的stx2转换噬菌体的多样性。
Microbiology (Reading). 2004 Sep;150(Pt 9):2959-2971. doi: 10.1099/mic.0.27188-0.
9
High pressure mediated lysogenic conversion of Escherichia coli with Shiga-toxin encoding bacteriophage.高压介导的大肠杆菌与携带志贺毒素编码噬菌体的溶原性转化
Commun Agric Appl Biol Sci. 2005;70(2):131-4.
10
Characterization of a Shiga toxin-encoding temperate bacteriophage of Shigella sonnei.宋内志贺氏菌产志贺毒素温和噬菌体的特性分析
Infect Immun. 2001 Dec;69(12):7588-95. doi: 10.1128/IAI.69.12.7588-7595.2001.
引用本文的文献
1
Rapid Enzymatic Detection of Shiga-Toxin-Producing Using Fluorescence-Labeled Oligonucleotide Substrates.使用荧光标记寡核苷酸底物快速酶促检测产志贺毒素菌
ACS Infect Dis. 2024 Dec 13;10(12):4103-4114. doi: 10.1021/acsinfecdis.4c00221. Epub 2024 Nov 22.
2
Evaluation of a direct phage DNA detection-based Taqman qPCR methodology for quantification of phage and its application in rapid ultrasensitive identification of Acinetobacter baumannii.基于直接噬菌体 DNA 检测的 Taqman qPCR 方法用于定量噬菌体及其在快速超敏鉴定鲍曼不动杆菌中的应用评估。
BMC Infect Dis. 2022 Jun 7;22(1):523. doi: 10.1186/s12879-022-07493-1.
3
Genetic and antimicrobial resistance profiles of non-O157 Shiga toxin-producing Escherichia coli from different sources in Egypt.埃及不同来源的非 O157 型产志贺毒素大肠杆菌的遗传和抗菌耐药谱。
BMC Microbiol. 2021 Sep 23;21(1):257. doi: 10.1186/s12866-021-02308-w.
4
Abundance and Expression of Shiga Toxin Genes in at the Recto-Anal Junction Relates to Host Immune Genes.在直肠-肛门交界处，志贺毒素基因的丰度和表达与宿主免疫基因有关。
Front Cell Infect Microbiol. 2021 Mar 17;11:633573. doi: 10.3389/fcimb.2021.633573. eCollection 2021.
5
Persistence of a Stx-Encoding Bacteriophage in Minced Meat Investigated by Application of an Improved DNA Extraction Method and Digital Droplet PCR.应用改进的DNA提取方法和数字液滴PCR研究stx编码噬菌体在碎肉中的持久性
Front Microbiol. 2021 Jan 20;11:581575. doi: 10.3389/fmicb.2020.581575. eCollection 2020.
6
Investigation of the Causes of Shigatoxigenic PCR Positive and Culture Negative Samples.产志贺毒素性PCR阳性且培养阴性样本的原因调查
Microorganisms. 2020 Apr 18;8(4):587. doi: 10.3390/microorganisms8040587.
7
Closed Genome and Comparative Phylogenetic Analysis of the Clinical Multidrug Resistant Shigella sonnei Strain 866.临床多重耐药宋内志贺菌 866 株的闭合基因组与系统进化比较分析
Genome Biol Evol. 2018 Sep 1;10(9):2241-2247. doi: 10.1093/gbe/evy168.
8
Complete Genome Sequence of Strain WG5.菌株WG5的全基因组序列
Genome Announc. 2018 Jan 11;6(2):e01403-17. doi: 10.1128/genomeA.01403-17.
9
Real-Time qPCR as a Method for Detection of Antibody-Neutralized Phage Particles.实时定量聚合酶链反应作为检测抗体中和噬菌体颗粒的一种方法。
Front Microbiol. 2017 Nov 6;8:2170. doi: 10.3389/fmicb.2017.02170. eCollection 2017.
10
Contribution of cropland to the spread of Shiga toxin phages and the emergence of new Shiga toxin-producing strains.耕地对志贺毒素噬菌体传播和新型志贺毒素产生菌株出现的贡献。
Sci Rep. 2017 Aug 10;7(1):7796. doi: 10.1038/s41598-017-08169-6.
本文引用的文献
1
Quantification of Shiga toxin-converting bacteriophages in wastewater and in fecal samples by real-time quantitative PCR.应用实时定量 PCR 技术对污水和粪便样本中志贺毒素转化噬菌体进行定量分析。
Appl Environ Microbiol. 2010 Sep;76(17):5693-701. doi: 10.1128/AEM.00107-10. Epub 2010 Jul 9.
2
Development and validation of a qPCR-based method for quantifying Shiga toxin-encoding and other lambdoid bacteriophages.基于 qPCR 的方法用于定量检测产志贺毒素编码噬菌体和其他λ噬菌体的开发与验证。
Environ Microbiol. 2010 May;12(5):1194-204. doi: 10.1111/j.1462-2920.2010.02162.x. Epub 2010 Feb 9.
3
High-throughput method for rapid induction of prophages from lysogens and its application in the study of Shiga Toxin-encoding Escherichia coli strains.高通量方法快速诱导溶源菌噬菌体及其在志贺毒素编码大肠杆菌菌株研究中的应用。
Appl Environ Microbiol. 2010 Apr;76(7):2360-5. doi: 10.1128/AEM.02923-09. Epub 2010 Feb 5.
4
Quantification of Shiga toxin 2-encoding bacteriophages, by real-time PCR and correlation with phage infectivity.实时 PCR 定量检测志贺毒素 2 编码噬菌体及其与噬菌体感染力的相关性。
J Appl Microbiol. 2010 Mar;108(3):1105-1114. doi: 10.1111/j.1365-2672.2010.04664.x. Epub 2010 Jan 11.
5
Pathogenic potential and horizontal gene transfer in ovine gastrointestinal Escherichia coli.绵羊胃肠道大肠杆菌的致病潜力和水平基因转移。
J Appl Microbiol. 2010 May;108(5):1552-62. doi: 10.1111/j.1365-2672.2009.04575.x. Epub 2009 Oct 7.
6
Phage-mediated Shiga toxin 2 gene transfer in food and water.噬菌体介导的志贺毒素2基因在食物和水中的转移
Appl Environ Microbiol. 2009 Mar;75(6):1764-8. doi: 10.1128/AEM.02273-08. Epub 2009 Jan 23.
7
Quantitative real-time PCR is not more sensitive than "conventional" PCR.定量实时聚合酶链反应并不比“传统”聚合酶链反应更灵敏。
J Clin Microbiol. 2008 Jun;46(6):1897-900. doi: 10.1128/JCM.02258-07. Epub 2008 Apr 9.
8
Differential expression of stx2 variants in Shiga toxin-producing Escherichia coli belonging to seropathotypes A and C.志贺毒素产生大肠杆菌中stx2变体在血清致病型A和C中的差异表达。
Microbiology (Reading). 2008 Jan;154(Pt 1):176-186. doi: 10.1099/mic.0.2007/009704-0.
9
Multilocus characterization scheme for shiga toxin-encoding bacteriophages.产志贺毒素噬菌体的多位点特征分析方案
Appl Environ Microbiol. 2007 Dec;73(24):8032-40. doi: 10.1128/AEM.01278-07. Epub 2007 Oct 19.
10
Insertion site occupancy by stx2 bacteriophages depends on the locus availability of the host strain chromosome.stx2噬菌体的插入位点占有率取决于宿主菌株染色体的基因座可用性。
J Bacteriol. 2007 Sep;189(18):6645-54. doi: 10.1128/JB.00466-07. Epub 2007 Jul 20.
Zotero 插件